Highly enantioselective and efficient synthesis of methyl (R)-o-chloromandelate with recombinant E. coli: toward practical and green access to clopidogrel.
Highly enantioselective and efficient synthesis of methyl (R)-o-chloromandelate with recombinant E. coli: toward practical and green access to clopidogrel.
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DOI：
10.1039/b703463f
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发表时间：
2007-04
影响因子：
3.2
通讯作者：
T. Ema;Nobuyasu Okita;Sayaka Ide;T. Sakai
中科院分区：
文献类型：
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作者：
T. Ema;Nobuyasu Okita;Sayaka Ide;T. Sakai
用重组大肠杆菌不对称还原邻氯苯甲酰基甲酸甲酯（2）（直至1.0 M），得到（R）-邻氯扁桃酸甲酯（（R）-），它是一种血小板聚集抑制剂氯吡格雷的中间体，ee>99%。大肠杆菌过量生产一种多功能羰基还原酶。
Methyl (R)-o-chloromandelate ((R)-), which is an intermediate for a platelet aggregation inhibitor named clopidogrel, was obtained in >99% ee by the asymmetric reduction of methyl o-chlorobenzoylformate (2) (up to 1.0 M) with recombinant E. coli overproducing a versatile carbonyl reductase.