Ribosome pausing, a dangerous necessity for co-translational events
Ribosome pausing, a dangerous necessity for co-translational events
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DOI：
10.1093/nar/gkz763
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发表时间：
2020-02-20
影响因子：
14.9
通讯作者：
Weiss, Benjamin
中科院分区：
文献类型：
--
作者：
Collart, Martine A.;Weiss, Benjamin
近年来，翻译伸长已成为调控基因表达的重要因素。在生产成熟蛋白质的过程中，有多个质量控制检查点，将它们定位到正确的细胞区室，或者将它们与它们的伴侣正确地联系起来。核糖体暂停以允许共翻译蛋白质折叠，蛋白质靶向或蛋白质相互作用，暂停是由mRNA序列和结构，tRNA可用性和新生肽的组合决定的。然而，核糖体暂停也会导致核糖体碰撞和mRNA和新生链的共翻译降解。了解翻译核糖体如何调整新生蛋白质必须经历的不同成熟步骤，这些成熟事件的时间是什么，以及在需要暂停时如何避免降解，现在有可能通过跟踪体内核糖体动力学的方法出现。这篇综述总结了一些最近的研究，这些研究提高了我们对利用核糖体谱分析的力量进行共翻译事件的认识，以及从这些研究中出现的一些问题。
In recent years translation elongation has emerged as an important contributor to the regulation of gene expression. There are multiple quality control checkpoints along the way of producing mature proteins and targeting them to the right cellular compartment, or associating them correctly with their partners. Ribosomes pause to allow co-translational protein folding, protein targeting or protein interactions, and the pausing is dictated by a combination of the mRNA sequence and structure, the tRNA availability and the nascent peptide. However, ribosome pausing can also lead to ribosome collisions and co-translational degradation of both mRNA and nascent chain. Understanding how the translating ribosome tunes the different maturation steps that nascent proteins must undergo, what the timing of these maturation events is, and how degradation can be avoided when pausing is needed, is now possible by the emergence of methods to follow ribosome dynamics in vivo. This review summarizes some of the recent studies that have advanced our knowledge about co-translational events using the power of ribosome profiling, and some of the questions that have emerged from these studies.