NOVEL INFRARED SPECTROSCOPIC METHOD FOR THE DETERMINATION OF CRYSTALLINITY OF HYDROXYAPATITE MINERALS
NOVEL INFRARED SPECTROSCOPIC METHOD FOR THE DETERMINATION OF CRYSTALLINITY OF HYDROXYAPATITE MINERALS
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DOI：
10.1016/s0006-3495(91)82113-0
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发表时间：
1991-10-01
影响因子：
3.4
通讯作者：
MENDELSOHN, R
中科院分区：
文献类型：
--
作者：
PLESHKO, N;BOSKEY, A;MENDELSOHN, R
利用傅里叶变换红外光谱（FT-IR）研究了生物学上重要的磷灰石类似物，并开发了一种定量评估其结晶度的方法。 用曲线拟合和二阶导数光谱分析了一系列晶体尺寸为100-200埃的合成磷灰石（含有氢氧根、氟离子或碳酸根离子）和生物磷灰石的磷酸盐nu-1和nu-3区域（900- 1，200 cm-1）的变化。 nu-1、nu-3谱线由三个主要的亚带组成。 两个光谱参数和晶体尺寸之间的相关性，通过X-射线衍射测定。 随着羟基磷灰石（HA）化合物c轴长度的增加，靠近1，060 cm-1的成分的面积百分比下降，而靠近1，020 cm-1的谱带的频率随着羟基磷灰石c轴长度的增加而增加。 因此，这些参数提出了生物（结晶不良）HA的结晶度指数。 对高结晶度磷灰石化合物的红外光谱进行了分析。 对于200-450埃的晶体尺寸，磷酸盐nu-1带（960 cm-1附近）的百分比面积随着HA晶体尺寸的增加而降低。 因此，结晶度的IR指数已开发为结晶良好和结晶不良的HA衍生物。 讨论了对Nu-1，Nu-3轮廓的各种贡献的分子起源，并说明了该方法对微观生物样品（大鼠骨骺生长板）的初步应用。
Biologically important apatite analogues have been examined by Fourier Transform Infrared Spectroscopy (FT-IR), and a method developed to quantitatively assess their crystallinity. Changes in the phosphate nu-1 and nu-3 regions, 900-1,200 cm-1, for a series of synthetic (containing hydroxide, fluoride, or carbonate ion) and biological apatites with crystal sizes of 100-200 angstrom were analyzed with curve-fitting and second derivative spectroscopy. The nu-1, nu-3 contour was composed of three main subbands. Correlations were noted between two spectral parameters and crystal size as determined by x-ray diffraction. The percentage area of a component near 1,060 cm-1 decreased as the length of the c-axis of the hydroxyapatite (HA) compounds increased, while the frequency of a band near 1,020 cm-1 increased with increasing length of the apatite c-axis. These parameters are thus proposed as indices of crystallinity for biological (poorly crystalline) HA. The FT-IR spectra of highly crystalline apatitic compounds were also analyzed. For crystal sizes of 200-450 angstrom, the percentage area of the phosphate nu-1 band (near 960 cm-1) decreased with increasing HA crystal size. IR indices of crystallinity have thus been developed for both well crystallized and poorly crystallized HA derivatives. The molecular origins of the various contributions to the nu-1, nu-3 contour are discussed, and a preliminary application of the method to a microscopic biological sample (rat epiphyseal growth plate) is illustrated.