THE HIV A (SOR) GENE-PRODUCT IS ESSENTIAL FOR VIRUS INFECTIVITY
THE HIV A (SOR) GENE-PRODUCT IS ESSENTIAL FOR VIRUS INFECTIVITY
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DOI：
10.1038/328728a0
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发表时间：
1987-08-20
期刊：
影响因子：
64.8
通讯作者：
MARTIN, MA
中科院分区：
文献类型：
--
作者：
STREBEL, K;DAUGHERTY, D;MARTIN, MA
人类免疫缺陷病毒（HIV）的基因组包含几个其他病毒中不存在的开放阅读框（ORF）。“A”基因1，也称为Q2 P '3、ORF-14或5，与polgene部分重叠;其蛋白产物的相对分子质量为23，000（Mr 23 K），存在于生产性感染的细胞中7 -10。该蛋白的功能尚不清楚;在“A”中缺失的突变病毒在CD 4+淋巴细胞系中复制并杀死CD 4+淋巴细胞系8，但在9种不同的HIV分离株中推导的氨基酸序列的高度保守性（80%）以及在HIV-211和其他慢病毒中存在类似基因表明该基因功能是重要的。在这里，我们描述了一个突变病毒缺陷的'A'基因，产生病毒粒子正常，但是，颗粒是小于1,000倍的感染性比野生型。反式互补实验部分恢复了感染性。然而，当病毒产生细胞与CD 4+淋巴细胞共培养时，突变病毒有效地传播，这表明HIV可以在细胞间传播，其机制不需要“A”基因产物，并且可能不需要产生感染性病毒颗粒。
The genome of the human immunodeficiency virus (HIV) contains several open reading frames (ORFs) not present in other viruses. The 'A' gene1, also known asQ2P'3,ORF-14orsor5, partially overlaps thepolgene; its protein product has a relative molecular mass of 23,000 (Mr23K) and is present in productively infected cells7–10. The function of this protein is unclear; mutant viruses deleted in 'A' replicate in and kill CD4+lymphocyte lines8, but the high degree of conservation of the deduced amino-acid sequence in nine different HIV isolates (80%) and the presence of analogous genes in HIV-211and other lentiviruses suggest that the gene function is an important one. Here we describe a mutant virus deficient in the 'A' gene which produces virion particles normally; however, the particles are & sm;1,000 times less infective than wild type. Transcomplementation experiments partially restore infectivity. The mutant virus spreads efficiently when virus-producing cells are co-cultivated with CD4+lymphocytes, however, indicating that HIV can spread from cell to cell in a mechanism that does not require the 'A' gene product and probably does not require the production of infective virus particles.