Quantitative and chemical fingerprint analysis for quality control of Rhizoma Coptidischinensis based on UPLC-PAD combined with chemometrics methods
Quantitative and chemical fingerprint analysis for quality control of Rhizoma Coptidischinensis based on UPLC-PAD combined with chemometrics methods
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DOI：
10.1016/j.phymed.2009.03.016
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发表时间：
2009-10-01
期刊：
影响因子：
7.9
通讯作者：
Li, Zu-Lun
中科院分区：
文献类型：
--
作者：
Kong, Wei-Jun;Zhao, Yan-Ling;Li, Zu-Lun
为控制黄连药材的质量，建立了基于超高效液相色谱-光电二极管阵列检测器(UPLC-PAD)的黄连药材中5种活性生物碱的定量分析和化学指纹图谱分析方法。在定量分析中，5种生物碱在试验范围内呈良好的线性关系(R>；0.999 2)，方法的回收率在98.4-100.8%之间。PAD中5种生物碱的检出限和定量限分别小于0.07和0.22微克/毫升。为了比较不同产地黄连的UPLC指纹图谱，应用化学计量学方法，包括相似性分析(SA)、层次聚类分析(HCA)、主成分分析(PCA)，对不同产地黄连样品进行了分类。结果表明，黄连药材可以成功地按产地进行归类。在此基础上，筛选出5个主要的化学标记成分，可用于黄连药材的准确鉴别和质量控制。结果表明，UPLC-PAD法是一种简便、灵敏、可靠的定量和化学指纹图谱分析方法，可用于黄连药材的质量评价和控制。(C)2009年爱思唯尔股份有限公司。版权所有。
To control the quality of Rhizoma Coptidis, a method based on ultra performance liquid chromatography with photodiode array detector (UPLC-PAD) was developed for quantitative analysis of five active alkaloids and chemical fingerprint analysis. In quantitative analysis, the five alkaloids showed good regression (R > 0.999 2) within test ranges and the recovery of the method was in the range of 98.4-100.8%. The limit of detections and quantifications for five alkaloids in PAD were less than 0.07 and 0.22 mu g/ml, respectively. In order to compare the UPLC fingerprints between Rhizoma Coptidis from different origins, the chemometrics procedures, including similarity analysis (SA), hierarchical clustering analysis (HCA), principal component analysis (PCA) were applied to classify the Rhizoma Coptidis samples according to their cultivated origins. Consistent results were obtained to show that Rhizoma Coptidis samples could be successfully grouped in accordance with the province of origin. Furthermore, five marker constituents were screened out to be the main chemical marker, which could be applied to accurate discrimination and quality control for Rhizoma Coptidis by quantitative analysis. This study revealed that UPLC-PAD method was simple, sensitive and reliable for quantitative and chemical fingerprint analysis, moreover, for the quality evaluation and control of Rhizoma Coptidis. (C) 2009 Elsevier GmbH. All rights reserved.