Synthesis of a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli
Synthesis of a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli
复制标题
产肠毒素大肠杆菌人菌株 SK-1 产生的热稳定肠毒素 (STh) 的合成
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
T. Miwatani
中科院分区：
文献类型：
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作者：
H. Ikemura;S. Yoshimura;S. Aimoto;Y. Shimonishi;S. Hara;T. Takeda;Y. Takeda;T. Miwatani
用常规方法合成了人产肠毒素大肠杆菌SK-1株产生的耐热肠毒素(STH)的氨基酸序列。合成Sth在0.8 ng剂量下可引起乳鼠体液分泌，与天然Sth的有效剂量相近。抗天然毒素抗血清可完全抑制小鼠的体液分泌。此外，合成的Sth具有与天然Sth相同的1H-核磁共振波谱和热稳定性。
A peptide with the amino acid sequence proposed for a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli was synthesized by a conventional method. Synthetic STh evoked fluid secretion in suckling mice at a dose of 0.8 ng, which is similar to the effective dose of native STh. The fluid secretion was completely inhibited by antisera raised against the native toxin. Furthermore, synthetic STh showed the same 1H-NMR spectrum and heat-stability as native STh.