C57BL/6 and 129/Sv mice: genetic difference to renal ischemia-reperfusion
C57BL/6 and 129/Sv mice: genetic difference to renal ischemia-reperfusion
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C57BL/6 和 129/Sv 小鼠：肾缺血再灌注的遗传差异
DOI：
10.5301/jn.5000053
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发表时间：
2012-09-01
影响因子：
3.4
通讯作者：
Rong, Song
中科院分区：
文献类型：
--
作者：
Lu, Xia;Li, Na;Rong, Song
背景资料：C57 BL/6和129/Sv是肾缺血再灌注损伤（IRI）研究中最常用的两种小鼠品系，但目前还没有对比缺血再灌注后肾损伤程度差异的研究。为了评价雄性C57 BL/6和129/Sv小鼠的肾IRI，行单侧肾蒂钳夹45分钟，比较肾组织损伤程度和肾功能。为了测量功能和组织损伤，我们检查：肾小球滤过率（GFR;菊糖清除率），肾血流量（RBF;由p-氨基马尿酸[PAH]清除率），肾形态，浸润性白细胞的免疫组织化学，和纤维化标记物天狼星红staining.Results：后单方面IRI，129/sv小鼠有显着减少GFR和RBF功能障碍在第14天（第14天）和第28天。129/sv小鼠在第1天急性肾小管坏死和第28天浸润的白细胞数量也明显少于C57 BL/6小鼠，第28天胶原沉积也少于C57 BL/6小鼠。
Background: C57BL/6 and 129/Sv are the 2 most commonly used strains of mice in renal ischemia-reperfusion injury (IRI) studies, yet there are currently no studies that contrast differences in the degree of renal injury after ischemia-reperfusion.Methods: To evaluate renal IRI in male C57BL/6 and 129/Sv mice, we performed unilateral clamping of the left renal pedicle for 45 minutes and compared the degree of renal tissue damage and function. To measure function and tissue damage we examined: glomerular filtration rate (GFR; by inulin clearance), renal blood flow (RBF; by p-aminohippurate [PAH] clearance), renal morphology, immunohistochemistry for infiltrating leukocytes, and fibrogenic markers by Sirius red staining.Results: After unilateral IRI, 129/sv mice had significantly less GFR and RBF disfunction at both day 14 (d14) and d28. 129/sv mice also had significantly less acute tubular necrosis on d1 and fewer infiltrating leukocytes on d28, as well as less collagen deposition on d28 than C57BL/6 mice.Conclusions: C57BL/6 mice were much more sensitive to damage caused by renal IRI than are 129/Sv mice.