Basso mouse scale for locomotion detects differences in recovery after spinal cord in ury in five common mouse strains
Basso mouse scale for locomotion detects differences in recovery after spinal cord in ury in five common mouse strains
复制标题
DOI：
10.1089/neu.2006.23.635
复制
发表时间：
2006-05-01
影响因子：
4.2
通讯作者：
Popovich, PG
中科院分区：
文献类型：
--
作者：
Basso, DM;Fisher, LC;Popovich, PG
基因工程小鼠被广泛用于检测脊髓损伤（SCI）的分子反应。固有的压力差异可能会混淆行为结果；因此，有必要对脊髓损伤后的几种菌株进行行为表征。Basso, Beattie， Bresnahan大鼠运动评定量表（BBB）已广泛用于脊髓损伤小鼠，但可能不能准确反映其独特的恢复模式。本研究的目的是建立一个有效的运动评定量表，并确定脊髓损伤后运动恢复的应变差异。我们检测了C57BL/6、C57BL/10、B10.PL、BALB/c和C57BL/6x129S6 F1菌株在轻度、中度和重度挫伤脊髓或中胸脊髓横断后42天的表现。挫伤是用俄亥俄州立大学的电磁SCI设备制造的，这是一个位移驱动的模型，无限地平线设备，这是一个力驱动的模型。Basso小鼠运动量表（BMS）的属性和排名是通过对七种运动类别的频率分析确定的。小鼠在协调性、爪位和躯干不稳定性方面的恢复与大鼠不同。在使用BBB （p < 0.05）而不是BMS评估同一只小鼠时，六名专家评分者出现了分歧。BMS检测到严重挫伤和横断之间的运动结果有显著差异（p < 0.05），而位移变化仅为0.1 mm的SCI严重程度分级（p < 0.05）。BMS具有显著的面部效度、预测效度和并发效度。经过培训的BMS评分新手得分在专家0.5分以内，具有较高的信度（0.92-0.99）。BMS是一种灵敏、有效、可靠的脊髓损伤小鼠运动测量方法。中度脊髓损伤后，BMS对C57BL/10、B10.PL和F1的恢复显著高于C57BL/6和BALB/c菌株（p < 0.05）。对脊髓损伤的不同行为反应表明，内在遗传因素显著影响运动恢复，必须在近亲繁殖或基因工程小鼠品系的研究中加以考虑。
Genetically engineered mice are used extensively to examine molecular responses to spinal cord injury (SCI). Inherent strain differences may confound behavioral outcomes; therefore, behavioral characterization of several strains after SCI is warranted. The Basso, Beattie, Bresnahan Locomotor Rating Scale (BBB) for rats has been widely used for SCI mice, but may not accurately reflect their unique recovery pattern. This study's purpose was to develop a valid locomotor rating scale for mice and to identify strain differences in locomotor recovery after SCI. We examined C57BL/6, C57BL/10, B10.PL, BALB/c, and C57BL/6x129S6 F1 strains for 42 days after mild, moderate, and severe contusive SCI or transection of the mid thoracic spinal cord. Contusions were created using the Ohio State University electromagnetic SCI device which is a displacement-driven model, and the Infinite Horizon device, which is a force-driven model. Attributes and rankings for the Basso Mouse Scale for Locomotion (BMS) were determined from frequency analyses of seven locomotor categories. Mouse recovery differed from rats for coordination, paw position and trunk instability. Disagreement occurred across six expert raters using BBB (p < 0.05) but not BMS to assess the same mice. BMS detected significant differences in locomotor outcomes between severe contusion and transection (p < 0.05) and SCI severity gradations resulting from displacement variations of only 0.1 mm (p < 0.05). BMS demonstrated significant face, predictive and concurrent validity. Novice BMS raters with training scored within 0.5 points of experts and demonstrated high reliability (0.92-0.99). The BMS is a sensitive, valid and reliable locomotor measure in SCI mice. BMS revealed significantly higher recovery in C57BL/10, B10.PL and F1 than the C57BL/6 and BALB/c strains after moderate SCI (p < 0.05). The differing behavioral response to SCI suggests inherent genetic factors significantly impact locomotor recovery and must be considered in studies with inbred or genetically engineered mouse strains.