Graphite-Coated Magnetic Nanoparticle Microarray for Few-Cells Enrichment and Detection
Graphite-Coated Magnetic Nanoparticle Microarray for Few-Cells Enrichment and Detection
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用于少细胞富集和检测的石墨涂层磁性纳米颗粒微阵列
DOI：
10.1021/nn2034692
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发表时间：
2012-02-01
期刊：
影响因子：
17.1
通讯作者：
Dai, Hongjie
中科院分区：
文献类型：
--
作者：
Chen, Zhuo;Hong, Guosong;Dai, Hongjie
采用化学气相沉积法合成了石墨包覆的高磁性FeCo核壳纳米粒子，并通过独特的聚合物混合物改性使其增溶于水溶液中，显著提高了磁性纳米粒子（MNP）的生物相容性和稳定性。这种功能化的MNP被证明在不同的条件下是非常稳定的，这对于生物应用是重要的。使用这些MNP开发了细胞染色、操作、富集和检测。在外部磁操纵下，MNP染色的细胞表现出定向运动。此外，MNP被印刷在基板上，以调制表面上的磁场分布。已经以微阵列方式探索了掺入全血中的癌细胞稀疏群体的捕获和检测。在MNP微阵列芯片上，能够从1 mL全血中简单有效地检测到数百个甚至两个癌细胞。有趣的是，通过MNP微阵列捕获的细胞在孵育后仍显示出活力并粘附于MNP点，这可用于癌细胞检测、局部生长和增殖。
Graphite-coated, highly magnetic FeCo core-shell nanoparticles were synthesized by a chemical vapor deposition method and solubilized in aqueous solution through a unique polymer mixture modification, which significantly Improved the biocompatibility and stability of the magnetic nanoparticles (MNPs). Such functionalized MNPs were proven to be very stable in different conditions which would be significant for biological applications. Cell staining, manipulation, enrichment, and detection were developed with these MNPs. Under external magnetic manipulation, the MNP-stained cells exhibited directed motions. Moreover, MNPs were printed on substrates to modulate the magnetic field distribution on the surface. Capture and detection of sparse populations of cancer cells spiked into whole blood has been explored in a microarray fashion. Cancer cells from hundreds down to only two were able to be simply and efficiently detected from 1 mL of whole blood on the MNP microarray chips. Interestingly, the cells captured through the MNP microarray still showed viability and adhered to the MNP spots after incubation, which could be utilized for cancer cell detection, localized growth, and proliferation.