A SIMPLE MODIFIED CARBODIIMIDE METHOD FOR CONJUGATION OF SMALL-MOLECULAR-WEIGHT COMPOUNDS TO IMMUNOGLOBULIN-G WITH MINIMAL PROTEIN CROSSLINKING
A SIMPLE MODIFIED CARBODIIMIDE METHOD FOR CONJUGATION OF SMALL-MOLECULAR-WEIGHT COMPOUNDS TO IMMUNOGLOBULIN-G WITH MINIMAL PROTEIN CROSSLINKING
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DOI：
10.1016/0003-2697(81)90380-8
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
PRESTON, JF
中科院分区：
文献类型：
--
作者：
DAVIS, MTB;PRESTON, JF
本文研究了用改进的碳二亚胺反应将低分子上的羧基偶联到兔免疫球蛋白G上的反应性氨基的最佳条件。[~(14)C]马尿酸与N-乙基-N‘-(二甲氨基丙基)碳二亚胺在pH=5下反应，然后调节到pH=8，再与兔免疫球蛋白偶联，形成的马尿酸-免疫球蛋白偶联物的分子内和分子间交联度均小于10%。马尿酸与免疫球蛋白结合后，93%以上的键对羟胺水解稳定，表明这些键具有多肽性质。这一两步法允许将一定数量的马尿酸部分负载到单个免疫球蛋白分子上，并为将含有羧基的分子连接到各种多肽上的氨基提供了一种有用的方法。
Optimal conditions for the conjugation of carboxyl groups on low molecular weight molecules to reactive amino groups on rabbit immunoglobulin G (IgG) using a modified carbodiimide reaction have been investigated. Reaction of [14C]hippuric acid with N-ethyl-N′-(dimethylaminopropyl) carbodiimide at pH 5 followed by adjustment to pH 8 and coupling with rabbit IgG resulted in the formation of hippuric acid-IgG conjugates with less than 10% intra- and intermolecular IgG crosslinking. More than 93% of the bonds linking hippuric acid to IgG were stable to hydroxylamine hydrolysis, indicating the peptide properties of these bonds. This two-step process permitted a defined number of hippuric acid moieties to be loaded onto a single IgG molecule and should provide a useful method for the conjugation of molecules containing carboxyl groups to amino groups on a variety of polypeptides.