Cross Breeding and Hybrid Identification of Sulphite-tolerant Hybrids of Saccharomyces uvarum
Cross Breeding and Hybrid Identification of Sulphite-tolerant Hybrids of Saccharomyces uvarum
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DOI：
10.21548/38-2-1044
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
H.Y. Zhang
中科院分区：
文献类型：
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作者：
X.Z. Liu;Z.M. Zhang;H.Y. Zhang
Saccharomyces uvarum is one of the yeast species with great potential for the wine industry. However, the sulfite tolerance of most S. uvarum strains is quite poor compared with the other Saccharomyces strains. In order to get excellent S. uvarum strains with tolerance to sulfite and also with good fermentation characteristics, 21 candidates were screened from three different crossing combinations of sensitive S. uvarum strains to one sulfite-tolerant strain. Ten of these hybrids were sulfite tolerant and contained the FZF1 gene from both parents, and inter-simple sequence repeat (ISSR) analysis confirmed their hybrid status, based on 6 primers that produced 55 clear and reproducible bands, including 32 that were polymorphic. Two hybrids had identical fingerprints consistent with their being the same clone. Selected hybrid candidate strains fermented very well at 30 ºC in Sauvignon Blanc grape juice containing 2 mM of sodium sulfite, with minor differences in fermentation performance. Among them, two strains (C13 and C21) performed very similarly to the sulfite-tolerant parent A9 and a commercial S. cerevisiae strain EC1118, and the production of fermentation aromas of C13 was found to be the highest. Thus, a total of nine different novel sulfite resistant hybrids of S. uvarum were obtained. This is the first report of using hybridization to breed the sulfur-tolerant S. uvarum strains.
