Genetic transformation of conifers and its application in forest biotechnology
Genetic transformation of conifers and its application in forest biotechnology
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针叶树遗传转化及其在森林生物技术中的应用
DOI：
10.1007/s00299-003-0670-1
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发表时间：
2003
影响因子：
6.2
通讯作者：
R. Newton
中科院分区：
文献类型：
--
作者：
Wei Tang;R. Newton
利用基因转移技术对针叶树进行遗传改良是当前林业生物技术研究的一个重要领域。两种基本的方法，粒子轰击和农杆菌介导的转化，已被用于针叶树。粒子轰击的使用已经在欧洲云杉、欧洲云杉、马里亚纳云杉和辐射松中产生了稳定的转基因植物。通过农杆菌介导的转化，已经从落叶落叶松、欧洲云杉、灰绿松、马里亚纳松、白松、火炬松和辐射松产生了转基因植物。农杆菌介导的转化具有比粒子轰击法更简单的整合模式和引入的DNA中有限的重排等优点。目前，针叶树的遗传转化已朝着提高生长速度、木材性质和质量、抗虫性、抗逆性和抗除草剂性等方向发展，这将推动林业进入生产力和质量的新时代。
Genetic modification of conifers through gene transfer technology is now an important field in forest biotechnology. Two basic methodologies, particle bombardment and Agrobacterium-mediated transformation, have been used on conifers. The use of particle bombardment has produced stable transgenic plants in Picea abies, P. glauca, P. mariana, and Pinus radiata. Transgenic plants have been produced from Larix decidua, Picea abies, P. glauca, P. mariana, Pinus strobus, P. taeda, and P. radiata via Agrobacterium-mediated transformation. Agrobacterium-mediated transformation has advantages over particle bombardment such as a simpler integration pattern and a limited rearrangement in the introduced DNA. At present, genetic transformation of conifers has been directed toward improving growth rate, wood properties and quality, pest resistance, stress tolerance, and herbicide resistance, which will drive forestry to enter a new era of productivity and quality.