Laminin-Coated Electrospun Regenerated Silk Fibroin Mats Promote Neural Progenitor Cell Proliferation, Differentiation, and Survival in vitro
Laminin-Coated Electrospun Regenerated Silk Fibroin Mats Promote Neural Progenitor Cell Proliferation, Differentiation, and Survival in vitro
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层粘连蛋白涂层电纺再生丝素蛋白垫促进神经祖细胞体外增殖、分化和存活
DOI：
10.3389/fbioe.2019.00190
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发表时间：
2019-08-06
影响因子：
5.7
通讯作者：
Sun, Shan
中科院分区：
文献类型：
--
作者：
Li, Guangfei;Chen, Kai;Sun, Shan
神经前体细胞移植是一种很有前途的中枢神经系统重建和再生技术。生物材料支架、框架和平台可以在体外支持NPC增殖和分化，以及在移植后充当临时细胞外基质。然而，生物材料的进一步应用需要改进的生物属性。丝素蛋白是由家蚕产生的一种蛋白质聚合物，在生物材料领域有着广泛的应用和研究。在这里，我们制备了对齐和随机静电纺丝再生SF（RSF）支架，并评估其对NPC生长的影响。首先，我们分离NPC，然后将其培养在层粘连蛋白包被的RSF垫或常规层粘连蛋白包被的盖玻片上用于细胞测定。我们发现，与对照组相比，对齐和随机RSF分别导致NPC增殖增加143.8 +/- 13.3%和156.3 +/-14.7%。接下来，我们研究了神经元分化，发现与对照相比，对齐和随机RSF分别导致神经元分化增加约93.2 +/-6.4%和3167.1 +/-4.8%。此外，我们测量了NPC的存活率，发现RSF促进NPC的存活，并且发现这三组之间没有差异。最后，研究了在RSF垫上培养的细胞中的信号通路在神经细胞分化中的贡献。我们的研究结果表明，RSF垫提供了一个功能性的微环境，并代表了一个有用的支架在神经工程研究的新策略的发展。
Neural progenitor cell (NPC) transplantation is a promising technique for central nervous system (CNS) reconstruction and regeneration. Biomaterial scaffolds, frameworks, and platforms can support NPC proliferation and differentiation in vitro as well as serve as a temporary extracellular matrix after transplantation. However, further applications of biomaterials require improved biological attributes. Silk fibroin (SF), which is produced by Bombyx mori, is a widely used and studied protein polymer for biomaterial application. Here, we prepared aligned and random eletrospun regenerated SF (RSF) scaffolds, and evaluated their impact on the growth of NPCs. First, we isolated NPCs and then cultured them on either laminin-coated RSF mats or conventional laminin-coated coverslips for cell assays. We found that aligned and random RSF led to increases in NPC proliferation of 143.8 +/- 13.3% and 156.3 +/- 14.7%, respectively, compared to controls. Next, we investigated neuron differentiation and found that the aligned and the random RSF led to increases in increase in neuron differentiation of about 93.2 +/- 6.4%, and 3167.1 +/- 4.8%, respectively, compared to controls. Furthermore, we measured the survival of NPCs and found that RSF promoted NPC survival, and found there was no difference among those three groups. Finally, signaling pathways in cells cultured on RSF mats were studied for their contributions in neural cell differentiation. Our results indicate that RSF mats provide a functional microenvironment and represent a useful scaffold for the development of new strategies in neural engineering research.