Identification and expression of the mammalian homologue of Bicaudal-C
Identification and expression of the mammalian homologue of Bicaudal-C
复制标题
DOI：
10.1016/s0925-4773(00)00568-2
复制
发表时间：
2001-03-01
影响因子：
2.6
通讯作者：
De Robertis, EM
中科院分区：
文献类型：
--
作者：
Wessely, O;Tran, U;De Robertis, EM
翻译激活和抑制在胚胎发育中基因表达的时空调控中发挥着重要作用。 Bicaudal-C 是一种 RNA 结合分子，据信在转录后水平发挥作用。由于后决定簇 oskar 的异位和过早翻译，果蝇中的功能丧失突变体影响前后模式。 Bicaudal-C 的爪蟾同源物是少数几个在异位显微注射时在没有中胚层诱导的情况下导致内胚层形成的分子之一。在这里，我们报告了 Bicaudal-C 的小鼠和人类同源物的序列和表达模式。人类基因位于染色体10q21.2上。使用原位杂交对小鼠进行表达分析，不仅在非洲爪蟾中检测到的结构域中检测到 Bicaudal-C 的表达。而且还发生在以前未报告的地区。与非洲爪蟾一样，小鼠 Bicaudal-C mRNA 存在于正在生长的卵母细胞（Hensen 节）中。和发育中的肾脏。此外，在后期，它在发育中的肠内胚层、软骨形成区域、胸膜腹膜衍生物、肺间质和卵巢基质中强烈表达。我们得出结论，小鼠 Bicaudal-C 是母体提供的基因产物，在哺乳动物细胞分化过程中受到严格调控。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
Translational activation and repression play an important role in the spatial-temporal regulation of gene expression in embryonic development. Bicaudal-C is an RNA-binding molecule believed to function at this post-transcriptional level. Loss-of-function mutants in Drosophila affect anterior-posterior patterning because of ectopic and premature translation of the posterior determinant oskar. The Xenopus homologue of Bicaudal-C is one of the few molecules that, when microinjected ectopically, results in endoderm formation in the absence of mesoderm induction. Here we report the sequence and expression pattern of the murine and human homologues of Bicaudal-C. The human gene is located on chromosome 10q21.2. Expression analysis in mouse using in situ hybridization detects expression of Bicaudal-C not only in domains detected in Xenopus. but also in previously unreported regions. As in Xenopus, mouse Bicaudal-C mRNA is found in the growing oocyte, Hensen's node. and the developing kidney. Additionally, at later stages it is strongly expressed in the developing gut endoderm, in areas of cartilage formation, in pleuro-peritoneal membrane derivatives, in lung mesenchyme, and in the stroma of the ovary. We conclude that mouse Bicaudal-C is a maternally provided gene product that is tightly regulated during mammalian cell differentiation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.