The product of the MLD gene is a member of the membrane fatty acid desaturase family: Overexpression of MLD inhibits EGF receptor biosynthesis
The product of the MLD gene is a member of the membrane fatty acid desaturase family: Overexpression of MLD inhibits EGF receptor biosynthesis
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DOI：
10.1021/bi970091l
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发表时间：
1997-06-10
期刊：
影响因子：
2.9
通讯作者：
Gill, GN
中科院分区：
文献类型：
--
作者：
Cadena, DL;Kurten, RC;Gill, GN
膜脂肪酸去饱和酶负责将双键插入脂肪酸的特定位置。我们克隆了人膜脂肪酸（脂质）去饱和酶基因家族的新成员MLD。 MLD 的衍生氨基酸序列包含三个共有基序：HX3H、HX2HH 和 HX2HHXFP，它们是一组膜脂肪酸去饱和酶的特征。 MLD 被预测为一种多跨膜蛋白，并且被发现可以用洗涤剂从颗粒部分中提取，但不能用盐或尿素提取。 MLD广泛表达于人体组织中，且定位于内质网。 MLD 与表皮生长因子 (EGF) 受体的共转染导致受体表达降低，但不影响血小板源性生长因子受体表达。 MLD 过表达抑制 EGF 受体的生物合成，表明脂肪酸去饱和酶在调节 EGF 受体的生物合成过程中可能发挥作用。
Membrane fatty acid desaturases are responsible for inserting double bonds into specific positions in fatty acids. We have cloned a new member of the human membrane fatty acid (lipid) desaturase gene family, MLD. The derived amino acid sequence of MLD contains three consensus motifs, HX3H, HX2HH, and HX2HHXFP, that are characteristic of a group of membrane fatty acid desaturases. MLD is predicted to be a multiple membrane-spanning protein and is found to be extractable from particulate fractions with detergent but not salt or urea. MLD is widely expressed in human tissues and is localized to the endoplasmic reticulum. Cotransfection of MLD with the epidermal growth factor (EGF) receptor resulted in decreased expression of the receptor but did not affect platelet-derived growth factor receptor expression. MLD overexpression inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor.