The effect of autologous mesenchymal stem cells on the biomechanics and histology of gel-collagen sponge constructs used for rabbit patellar tendon repair
The effect of autologous mesenchymal stem cells on the biomechanics and histology of gel-collagen sponge constructs used for rabbit patellar tendon repair
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DOI：
10.1089/ten.2006.12.369
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发表时间：
2006-02-01
影响因子：
--
通讯作者：
Butler, DL
中科院分区：
文献类型：
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作者：
Juncosa-Melvin, N;Boivin, GP;Butler, DL
本研究的目的是将间充质干细胞（MSC）引入凝胶-海绵复合材料中，并检查细胞对术后12周修复生物力学和组织学的影响。我们测试了两个相关的假设-添加MSC将显着改善修复生物力学和细胞组织，并会导致更高的失败力比峰值在体内髌腱（PT）的力量记录的倾斜跳跃活动。通过将来自15只成年兔的MSC以0.1 × 10（6）个细胞/mL接种在胶原海绵中的2.6 mg/mL胶原凝胶中来创建自体组织工程构建体。在匹配的胶原海绵中使用相同浓度的胶原凝胶来创建脱细胞构建体。这些细胞和脱细胞结构被植入双侧髌腱中央三分之一处的全层全长缺损中。术后12周，修复组织被分配用于生物力学（n = 12对）和组织学（n = 3对）分析。细胞修复的最大力和最大应力分别约为PT正常中央三分之一相应值的60%和50%。同样，这些细胞修复的线性刚度和线性模量平均分别为正常PT值的75%和30%。相比之下，脱细胞修复体表现出最大力（40%）、最大应力（25%）、线性刚度（30%）和线性模量（20%）的正常PT值百分比较低。组织学上，两种修复都显示出III型和V型胶原蛋白、纤连蛋白和核心蛋白聚糖的强染色。细胞修复也显示出与正常肌腱相当的细胞排列。这项研究表明，将自体间充质干细胞引入凝胶-胶原海绵复合材料中，与在体内负荷范围内单独使用凝胶-海绵复合材料相比，显著改善了肌腱修复。
The objective of this study was to introduce mesenchymal stem cells (MSCs) into a gel-sponge composite and examine the effect the cells have on repair biomechanics and histology 12 weeks postsurgery. We tested two related hypotheses - adding MSCs would significantly improve repair biomechanics and cellular organization, and would result in higher failure forces than peak in vivo patellar tendon ( PT) forces recorded for an inclined hopping activity. Autogenous tissue-engineered constructs were created by seeding MSCs from 15 adult rabbits at 0.1 x 10(6) cells/mL in 2.6 mg/mL of collagen gel in collagen sponges. Acellular constructs were created using the same concentration of collagen gel in matching collagen sponges. These cellular and acellular constructs were implanted in bilateral full-thickness, full-length defects in the central third of patellar tendons. At 12 weeks after surgery, repair tissues were assigned for biomechanical ( n = 12 pairs) and histological ( n = 3 pairs) analyses. Maximum force and maximum stress for the cellular repairs were about 60 and 50% of corresponding values for the normal central third of the PT, respectively. Likewise, linear stiffness and linear modulus for these cellular repairs averaged 75 and 30% of normal PT values, respectively. By contrast, the acellular repairs exhibited lower percentages of normal PT values for maximum force (40%), maximum stress (25%), linear stiffness ( 30%), and linear modulus (20%). Histologically, both repairs showed strong staining for collagen types III and V, fibronectin, and decorin. The cellular repairs also showed cellular alignment comparable to that of normal tendon. This study shows that introducing autogenous mesenchymal stem cells into a gel-collagen sponge composite significantly improves tendon repair compared to the use of a gel-sponge composite alone in the range of in vivo loading.