A rapid and sensitive liquid chromatography/tandem mass spectrometry method for determination of docetaxel in human plasma
A rapid and sensitive liquid chromatography/tandem mass spectrometry method for determination of docetaxel in human plasma
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DOI：
10.1002/rcm.1091
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发表时间：
2003-01-01
影响因子：
2
通讯作者：
Lee, HS
中科院分区：
文献类型：
--
作者：
Wang, LZ;Goh, BC;Lee, HS
以紫杉醇为内标物，建立了一种新的、快速、灵敏的等度液相色谱/串联质谱（LC/MS/MS）法测定人血浆中多西他赛浓度。MS/MS检测的高灵敏度和特异性使得能够使用小体积的血浆（0.05 mL）和简单的液-液萃取程序。此外，非常短的运行时间（3 min）满足了大规模临床研究中监测多西他赛血浆水平的需求。多西他赛的校准曲线在5-1000 ng/mL范围内呈线性，相关系数>0.999，仅使用0.05 mL血浆。分析的日内和日间精密度（CV）为
A novel, rapid and sensitive isocratic liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for quantification of docetaxel in human plasma with paclitaxel as internal standard. The high sensitivity and specificity of MS/MS detection enabled the use of a small volume of plasma (0.05 mL) and a simple liquid-liquid extraction procedure. Furthermore, a very short run-time (3 min) fulfilled the need for monitoring plasma levels of docetaxel from large-scale clinical studies. The calibration curve for docetaxel was linear over the range 5-1000 ng/mL with coefficients of correlation >0.999 using only 0.05 mL, plasma. The intra- and inter-day precisions (CV) of analysis were