Diversity and evolution of multiple orc/cdc6-adjacent replication origins in haloarchaea.
Diversity and evolution of multiple orc/cdc6-adjacent replication origins in haloarchaea.
复制标题
盐古菌中多个 orc/cdc6 相邻复制起点的多样性和进化。
DOI：
10.1186/1471-2164-13-478
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发表时间：
2012-09-14
期刊：
影响因子：
4.4
通讯作者：
Xiang H
中科院分区：
文献类型：
--
作者：
Wu Z;Liu H;Liu J;Liu X;Xiang H
虽然在古细菌中观察到多个复制起点，但对其进化过程知之甚少。在这里，我们进行了预测（部分证明）的orc/cdc 6相关的复制起点在15个完全测序的盐古菌基因组的比较分析，调查嗜盐古菌复制起点的多样性和进化。多个orc/cdc 6相关的复制起点，预测在所有的分析haloarchaeal基因组的推定ORB（起源识别框），与orc/cdc 6基因的鉴定。这些预测的复制起点在Haloarcula hispanica的实验证实，通过自主复制活动。引人注目的是，有几个预测的复制起点在H。hispanica和Haloarcula marismortui位于其高度同源染色体的不同区域，表明这些复制起点可能是作为新基因组内容的一部分引入的。与起源相关的Orc/Cdc 6同源物和相应的预测ORB元件的比较显示，在一个给定的盐古菌的复制起点是相当多样的，而不同的盐古菌可以共享一些保守的起源。系统发育和基因组背景分析表明，有一个原始的复制起点（oriC 1），这是从古细菌的祖先继承，和其他几个起源可能演变和/或易位内的盐古细菌物种。这项研究提供了详细的信息，多个orc/cdc 6相关的复制起点的多样性，在盐古菌基因组，并提供了新的见解多个复制起点的进化在古菌。
While multiple replication origins have been observed in archaea, considerably less is known about their evolutionary processes. Here, we performed a comparative analysis of the predicted (proved in part) orc/cdc6-associated replication origins in 15 completely sequenced haloarchaeal genomes to investigate the diversity and evolution of replication origins in halophilic Archaea. Multiple orc/cdc6-associated replication origins were predicted in all of the analyzed haloarchaeal genomes following the identification of putative ORBs (origin recognition boxes) that are associated with orc/cdc6 genes. Five of these predicted replication origins in Haloarcula hispanica were experimentally confirmed via autonomous replication activities. Strikingly, several predicted replication origins in H. hispanica and Haloarcula marismortui are located in the distinct regions of their highly homologous chromosomes, suggesting that these replication origins might have been introduced as parts of new genomic content. A comparison of the origin-associated Orc/Cdc6 homologs and the corresponding predicted ORB elements revealed that the replication origins in a given haloarchaeon are quite diverse, while different haloarchaea can share a few conserved origins. Phylogenetic and genomic context analyses suggested that there is an original replication origin (oriC1) that was inherited from the ancestor of archaea, and several other origins were likely evolved and/or translocated within the haloarchaeal species. This study provides detailed information about the diversity of multiple orc/cdc6-associated replication origins in haloarchaeal genomes, and provides novel insight into the evolution of multiple replication origins in Archaea.
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影响因子：
3.2
作者：
Berquist, BR;DasSarma, S
通讯作者：
DasSarma, S
西班牙盐弧菌的完整基因组序列，用于研究遗传学、代谢和病毒-宿主相互作用的盐古菌模型
影响因子：
3.2
作者：
Liu, Hailong;Wu, Zhenfang;Xiang, Hua
通讯作者：
Xiang, Hua
DOI：
10.1073/pnas.0806414105
发表时间：
2008-10-28
影响因子：
11.1
作者：
Duggin, Iain G.;McCallum, Simon A.;Bell, Stephen D.
通讯作者：
Bell, Stephen D.
影响因子：
56.9
作者：
Dueber, Erin L. Cunningham;Corn, Jacob E.;Berger, James M.
通讯作者：
Berger, James M.
影响因子：
10.5
作者：
Ge, Xin Quan;Jackson, Dean A;Blow, J Julian
通讯作者：
Blow, J Julian