Nature of DNA repair synthesis resistant to inhibitors of polymerase alpha in human cells.
Nature of DNA repair synthesis resistant to inhibitors of polymerase alpha in human cells.
复制标题
DNA 修复合成的性质对人类细胞中聚合酶 α 抑制剂具有抵抗力。
DOI：
10.1021/bi00302a008
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Okumoto,DS
中科院分区：
文献类型：
--
作者：
Smith,CA;Okumoto,DS
材料和方法细胞培养。对于本文报道的许多实验，我们使用了命名为T98 G的人连续细胞系，其来源于多形性胶质母细胞瘤肿瘤（Stein，1979）。这些细胞的DNA含量约为二倍体细胞的3倍，并生长至高细胞密度，但与其他连续细胞系不同，它们表现出正常人二倍体成纤维细胞的细胞密度和血清依赖性G1期阻滞（接触抑制）特征。这些属性有助于这里使用的分析。T98 G细胞，GM 38（Institute for Medical Research，Camden，NJ;获自EC Friedberg，斯坦福大学医学中心）和IMR 90正常成纤维细胞（ATCC CCL 186;获自R. Schimke，this department）培养在Eagle'sbasal medium中，补充有10%胎牛血清和抗生素（Grand Island Biological Co.）在37 C、潮湿的CO2气氛中。每7天以1：4的比例传代培养T98 G细胞的储备培养物。对于预标记，将它们以1：4的比例分开，并在0.4或0.8 μ Ci/mL [32 P]-[32 P]中生长7天。
Materials and Methods Cell Culture. For many of the experiments reported here, we used a human continuous cell line designated T98G, derived from a glioblastoma multiforma tumor (Stein, 1979). These cells have about 3 times the DNA content of diploid cells and grow to high cell densities, but unlike other continuous lines, they exhibit the cell density and serum-dependent G1 arrest (contact inhibition) characteristic of normalhuman diploid fibroblasts. These properties facilitate the analysesused here. In several instances, we also used normal human diploid fi-broblasts.The T98G cells, GM38 (Institute for Medical Research, Camden, NJ; obtained from EC Friedberg, Stanford University Medical Center) and IMR90 normal fibroblasts (ATCC CCL186; obtained from R. Schimke, this department), were cultured in Eagle’sbasal medium supplemented with 10% fetal bovine serum and antibiotics (Grand Island Biological Co.) at 37 C in a humidified C02 atmosphere. Stock cultures of T98G cells were subcultivated at a ratio of 1: 4 every 7 days. For prelabeling, they were split at a ratio of 1: 4 and grown for 7 days in 0.4 or 0.8/iCi/mL [32P]-