A possible complex containing RNA processing enzymes.
A possible complex containing RNA processing enzymes.
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可能含有 RNA 加工酶的复合物。
DOI：
10.1016/0006-291x(82)91777-6
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发表时间：
1982
影响因子：
3.1
通讯作者：
Apirion,D
中科院分区：
文献类型：
--
作者：
Jain,SK;Pragai,B;Apirion,D
三种酶，RNA酶III，RNA酶E和RNA酶P参与大肠杆菌中RNA前体的加工。在含有突变的RNA酶III或RNA酶E的提取物中，在某些条件下RNA酶P活性不表达，而在野生型提取物中RNA酶P活性表达。在高速离心来自E.菌株的细胞提取物时，在含有所有这三种酶的大肠杆菌中，大多数RNA酶P、RNA酶III和RNA酶E活性以比它们已知尺寸重的颗粒沉积。在细胞提取物的蔗糖密度梯度中，部分RNA酶E和RNA酶P活性共沉淀，而大部分RNA酶III活性被发现朝向梯度的顶部。这种行为与其他核糖核酸酶如RNA酶II和RNA酶H不同，它们不作为复合物沉淀。这种复合物似乎不仅仅是由酶与核糖体的结合引起的。
The three enzymes, RNAase III, RNAase E and RNAase P participate in the processing of RNA precursors in Escherichia coli. In extracts which contain a mutated RNAase III or RNAase E under certain conditions RNAase P activity is not expressed while in the wild-type extract it is. Upon high-speed centrifugation of a cell extract from a strain of E., coli, which contains all these three enzymes, the majority of RNAase P, RNAase III and RNAase E activities sediment as particles heavier than their known sizes. In a sucrose density gradient of the cell extract, part of RNAase E and RNAase P activities co-sediment while most of the RNAase III activity is found toward the top of the gradient. This behavior is distinct from other ribonucleases such as RNAase II and RNAase H, which do not sediment as complexes. This complex does not seem to be caused merely by the association of the enzymes with ribosomes.