Genome-wide expression monitoring in Saccharomyces cerevisiae
Genome-wide expression monitoring in Saccharomyces cerevisiae
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DOI：
10.1038/nbt1297-1359
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发表时间：
1997-12-01
影响因子：
46.9
通讯作者：
Lockhart, DJ
中科院分区：
文献类型：
--
作者：
Wodicka, L;Dong, HL;Lockhart, DJ
芽殖酵母酿酒酵母的基因组序列已被用于设计和合成高密度寡核苷酸阵列，用于监测几乎所有酵母基因的表达水平。这种直接和高度平行的方法涉及总mRNA群体与一组四个阵列的杂交，所述阵列含有总共超过260，000个使用光定向组合化学原位合成的特定选择的寡核苷酸。测量是定量的、灵敏的、特异的和可再现的。对于在丰富培养基和基本培养基中生长的细胞，已经测量了每个细胞从小于0.1个拷贝到几百个拷贝的表达水平。所有酵母mRNA的近90%被观察到在两种条件下都存在，其中约50%以每个细胞0.1至1个拷贝的水平存在。在这些条件下观察到的许多差异表达的基因是预期的，但是对于许多先前未表征的基因也观察到了很大的差异。
The genomic sequence of the budding yeast Saccharomyces cerevisiae has been used to design and synthesize high-density oligonucleotide arrays for monitoring the expression levels of nearly all yeast genes. This direct and highly parallel approach involves the hybridization of total mRNA populations to a set of four arrays that contain a total of more than 260,000 specifically chosen oligonucleotides synthesized in situ using light-directed combinatorial chemistry. The measurements are quantitative, sensitive, specific, and reproducible. Expression levels ranging from less than 0.1 copies to several hundred copies per cell have been measured for cells grown in rich and minimal media. Nearly 90% of all yeast mRNAs are observed to be present under both conditions, with approximately 50% present at levels between 0.1 and 1 copy per cell. Many of the genes observed to be differentially expressed under these conditions are expected, but large differences are also observed for many previously uncharacterized genes.