A POTENTIAL 2ND MESSENGER ROLE FOR UNSATURATED FATTY-ACIDS - ACTIVATION OF CA-2+-DEPENDENT PROTEIN-KINASE
A POTENTIAL 2ND MESSENGER ROLE FOR UNSATURATED FATTY-ACIDS - ACTIVATION OF CA-2+-DEPENDENT PROTEIN-KINASE
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DOI：
10.1126/science.6231726
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
SNYDERMAN, R
中科院分区：
文献类型：
--
作者：
MCPHAIL, LC;CLAYTON, CC;SNYDERMAN, R
花生四烯酸酯和其他不饱和长链脂肪酸激活了来自人中性粒细胞的蛋白激酶C。花生四烯酸酯对激酶的激活需要钙，二油醇能增强激酶的激活，但不需要外源磷脂酰丝氨酸。在磷脂酰丝氨酸激活过程中，亚极大水平的花生四烯酸也增强了激酶对Ca的亲和力。因此，在许多细胞类型中，由配体-受体相互作用触发的花生四烯酸酯的释放可以通过激活蛋白激酶C在调节细胞功能中发挥第二信使作用。
Arachidonate and other unsaturated long-chain fatty acids activated protein kinase C from human neutrophils. Kinase activation by arachidonate required Ca and was enhanced by diolein, but it did not require exogenous phosphatidylserine. Submaximal levels of arachidonate also enhanced the affinity of the kinase for Ca during activation by phosphatidylserine. Thus the release of arachidonate, which is triggered in many cell types by ligand-receptor interactions, could play a second messenger role in the regulation of cellular function by activation of protein kinase C.