Germline‐specific Antigens Identified by Monoclonal Antibodies in the Nematode Caenorhabditis elegans 1
Germline‐specific Antigens Identified by Monoclonal Antibodies in the Nematode Caenorhabditis elegans 1
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单克隆抗体鉴定线虫秀丽隐杆线虫种系特异性抗原 1
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
J. Miwa
中科院分区：
文献类型：
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作者：
Y. Yamaguchi;K. Murakami;M. Furusawa;J. Miwa
27个单克隆抗体鉴定反应，通过间接免疫荧光抗体染色，与线虫的特异性细胞和组织的秀丽隐杆线虫，我们在这里报告三个单克隆抗体有关的性腺组织。一种抗体定义了一种抗原，该抗原在早期发育时分布在整个胚胎上，后来成为性腺（包括成熟卵母细胞）所特有的抗原。另外两个识别的抗原不对称地分布在受精卵细胞质的后部区域，注定成为生殖系前体细胞。每个抗原是连续分离的发育中的胚胎的生殖系前体细胞和，postembryonically，是唯一定位于周围的幼虫和成人的生殖系细胞核。
Of 27 monoclonal antibodies identified to react, by indirect immunofluorescent antibody staining, with specific cells and tissues of the nematode Caenorhabditis elegans, we report here three monoclonal antibodies pertaining to the gonadal tissues. One antibody defines an antigen that is distributed over the entire embryo at earlier development and later becomes unique to the gonad, including mature oocytes. The antigens recognized by the other two are distributed asymmetrically in the posterior region of the fertilized egg's cytoplasm destined to become the germline precursor cell. Each antigen is successively segregated only to the germline precursor cells of the developing embryo and, postembryonically, is uniquely localized around the germline cell nuclei of the larvae and adults.