EFFECTS OF SOLVENTS AND DETERGENTS ON THE CONTRACTIONS OF ISOLATED SMOOTH-MUSCLE PREPARATIONS
EFFECTS OF SOLVENTS AND DETERGENTS ON THE CONTRACTIONS OF ISOLATED SMOOTH-MUSCLE PREPARATIONS
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DOI：
10.1111/j.2042-7158.1990.tb07036.x
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发表时间：
1990-12-01
影响因子：
3.3
通讯作者：
FREY, HH
中科院分区：
文献类型：
--
作者：
BRAAK, K;FREY, HH
在离体器官上测试难溶性物质时，使用有机溶剂和增溶剂来增加水溶性。 为了便于选择适当的物质，这些化学品中的11个的影响进行了研究，在以下分离的平滑肌制备：豚鼠回肠刺激卡巴胆碱，组胺，5-HT或单场刺激;大鼠胃底刺激5-HT;和小鼠输精管刺激去甲肾上腺素或列车的场刺激。 检查了9种溶剂（丙酮、二乙二醇单乙醚、二甲亚砜、乙醇、甘油、甲醇、聚乙二醇400、1，2-丙二醇、四甘醇（四氢糠醇聚乙二醇醚））和2种去污剂（Triton-X 100和吐温80）。 输精管被证明是最具抵抗力的，而大鼠眼底和豚鼠回肠更敏感的溶剂的影响时，从1到10克L-1。 大多数溶剂引起非特异性、浓度依赖性可逆性收缩抑制。 高浓度的二甲基亚砜增加豚鼠回肠的收缩反应由5-HT刺激，并在两个实验与电刺激。 聚乙二醇400增强小鼠输精管对电刺激的反应。 总体而言，1，2-丙二醇和聚乙二醇400的影响最小，可以使用的浓度为3克L-1，在定性研究中，甚至高达10克L-1。 甘油，一元醇和二甲亚砜产生更强烈的影响，不应超过1-3克L-1的浓度。 二甘醇单乙醚、丙酮和四甘醇对缓蚀作用较强，其浴槽浓度不宜超过0.5 ~ 1g L ~（-1）。 在洗涤剂中，只有吐温80适合作为体外平滑肌制剂中的增溶剂，在本研究中，在10 mg L-1的离体器官耐受的浓度下形成胶束。
In testing poorly soluble substances in-vitro on isolated organs, organic solvents and solubilizers are used to increase water-solubility. To facilitate selection of appropriate substances, the effects of eleven of these chemicals have been studied in the following isolated smooth muscle preparations: guinea-pig ileum stimulated by carbachol, histamine, 5-HT or single field stimuli; rat fundus stimulated by 5-HT; and mouse vas deferens stimulated by noradrenaline or trains of field stimuli. Nine solvents (acetone, diethyleneglycol monoethylether, dimethyl sulphoxide, ethanol, glycerol, methanol, polyethylene glycol 400, 1,2-propanediol, Tetraglycol (tetrahydrofurfurylalcohol polyethyleneglycolether) and two detergents (Triton-X 100 and Tween 80) were examined. The vas deferens proved to be most resistent, whereas rat fundus and guinea-pig ileum were more sensitive to the effects of solvent when present from 1 to 10 g L-1. Most solvents caused non-specific, concentration-dependent reversible inhibition of contractions. Dimethyl sulphoxide in high concentrations increased the contractile responses of guinea-pig ileum stimulated by 5-HT and in both experiments with electrical stimulation. Polyethylene glycol 400 augmented the response of mouse vas deferens to electrical stimulation. Overall, 1,2-propylene glycol and polyethylene glycol 400 had the least effect and can be used in a concentration of 3 g L-1, and in qualitative studies even up to 10 g L-1. Glycerol, both monohydric alcohols and dimethyl sulphoxide produced more intense effects and should not exceed concentrations of 1-3 g L-1. Stronger inhibition was caused by diethyleneglycol monoethylether, acetone and Tetraglycol, and the bath concentrations of these substances should not exceed 0.5-1g L-1. Of the detergents only Tween 80 is suitable as a solubilizer in smooth muscle preparations in-vitro, forming micelles at 10 mg L-1 a concentration tolerated by isolated organs in this study.