Lysobacter prati sp. nov., isolated from a plateau meadow sample
Lysobacter prati sp. nov., isolated from a plateau meadow sample
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DOI：
10.1007/s10482-020-01386-6
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发表时间：
2020-01-24
影响因子：
2.6
通讯作者：
Li, Wen-Jun
中科院分区：
文献类型：
--
作者：
Fang, Bao-Zhu;Xie, Yuan-Guo;Li, Wen-Jun
从四川省红原县高原草甸土样中分离到一株新的变形菌，命名为SYSU H10001（T）。采用多相分类法对该菌株的分类地位进行了研究。16 S rRNA基因序列相似性分析和系统发育树分析表明，SYSU H10001（T）与Lysobacter soli KCTC 22011（T）和Lysobacter panacisoli JCM 19212（T）的同源性分别为98.6%和98.2%。基于基因组的次生代谢产物预测结果表明，菌株SYSU H10001（T）含有3簇细菌素、1簇非核糖体肽合成酶、1簇1型聚酮合成酶和1簇芳基多烯。此外，主要的类异戊二烯醌是Q-8，并且主要的脂肪酸被鉴定为iso-C-15：0、iso-C-17：0和总和特征9。极性脂质含有二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和三种未鉴定的磷脂。菌株SYSU H10001（T）基因组DNA G + C含量为66.5%（基因组）。基于表型、基因型和系统发育数据，菌株SYSU H10001（T）代表溶杆菌属的一个新种，建议将其命名为Lysobacter prati sp. nov.。模式菌株为SYSU H10001（T）（= KCTC 72062（T）= CGMCC 1.16662（T））。
A novel proteobacterial strain designated SYSU H10001(T) was isolated from a soil sample collected from plateau meadow in Hongyuan county, Sichuan province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU H10001(T) was most closely related to Lysobacter soli KCTC 22011(T) (98.6%, sequence similarity) and Lysobacter panacisoli JCM 19212(T) (98.2%). The prediction result of secondary metabolites based on genome shown that the strain SYSU H10001(T) contained 3 clusters of bacteriocins, 1 cluster of non-ribosomal peptide synthetase, 1 cluster of type 1 polyketide synthase and 1 cluster of arylpolyene. In addition, the major isoprenoid quinone was Q-8 and the major fatty acids were identified as iso-C-15:0, iso-C-17:0 and Summed feature 9. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and three unidentified phospholipids. The genomic DNA G + C content of strain SYSU H10001(T) was 66.5% (genome). On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU H10001(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter prati sp. nov. is proposed. The type strain is SYSU H10001(T) (= KCTC 72062(T) = CGMCC 1.16662(T)).