Introducing ortho-methoxyl group as a fluorescence-enhancing and bathochromic-shift bi-functional strategy for typical cysteine sensors
Introducing ortho-methoxyl group as a fluorescence-enhancing and bathochromic-shift bi-functional strategy for typical cysteine sensors
复制标题
引入邻甲氧基作为典型半胱氨酸传感器的荧光增强和红移双功能策略
DOI：
10.1016/j.talanta.2020.121217
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发表时间：
2020
期刊：
影响因子：
6.1
通讯作者：
Zhu Hai-Liang
中科院分区：
文献类型：
--
作者：
Yuan Zeng-Hui;Zhang Xu-Ping;Guan Jing;Chen Li-Li;Li Shu-Kai;Liu Ming;Qin Ya-Juan;Yang Yu-Shun;Zhu Hai-Liang
探索了一种实用的引入邻甲氧基的策略，实现了荧光增强和红移双功能的优化。在ISOPH-X系列半胱氨酸传感器中进行了测试，从而获得了成功的案例ISOPH-2。它以一种简单、兼容的方式实现了优化。通过对检测点的确认，基本确立了相应的检测策略，包括适用稳定性（24 h以上）、pH范围宽（7.0-9.0）、响应快（20 min）、生物相容性好、灵敏度高（LOD = 0.072 nm）、选择性高，以及对活细胞中Cys和C的生物监测。优雅的在这项工作中，邻甲氧基的引入策略导致了15 nm的红移和近4倍的荧光增强。该策略可以与双键引入方法相结合。与已报道的策略相比，通过打破红移和强荧光强度之间的困境，该策略可能为开发具有更多荧光团的更好传感器及其靶点机制提供有益信息。
A practical strategy of introducingortho-methoxyl group was explored to achieve the fluorescence-enhancing and bathochromic-shift bi-functional optimization. It was tested in the Cys sensing ISOPH-X series, thus the successful case, ISOPH-2, was obtained. It realized the optimization in a simple and compatible way. The corresponding strategy was basically established during the confirmation of checkpoints including applicable steadiness (over 24 h), wide pH range (7.0–9.0), rapid response (20 min), good biocompatibility, high sensitivity (LOD = 0.072 nm), high selectivity and biological monitoring of Cys in living cells as well asC. elegans. In this work, theo-methoxyl introduction strategy led to a 15 nm red shift and a near 4-fold fluorescence enhancement. This strategy could be combined with the double bond-introducing approach. Compared with reported strategies, by breaking the dilemma between red shift and strong fluorescent intensity, this strategy might offer beneficial information for exploiting better sensors with more fluorophores and mechanisms for their targets.