The Toxoplasma gondii calcium-dependent protein kinase 7 is involved in early steps of parasite division and is crucial for parasite survival.
The Toxoplasma gondii calcium-dependent protein kinase 7 is involved in early steps of parasite division and is crucial for parasite survival.
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DOI：
10.1111/cmi.12186
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发表时间：
2014-01
影响因子：
3.4
通讯作者：
Daher W
中科院分区：
文献类型：
--
作者：
Morlon-Guyot J;Berry L;Chen CT;Gubbels MJ;Lebrun M;Daher W
顶复门寄生虫表达各种钙依赖性蛋白激酶（CDPK），其中一些在入侵和流出中发挥重要作用。大多数顶复门中保守的 6 个 CDPK 中的 5 个已在疟原虫物种和/或弓形虫寄生虫中在分子和细胞水平上进行了研究，但 CDPK7 的功能迄今为止尚未得到表征。在弓形虫中，在细胞内复制过程中，通过称为内生作用的独特过程在母细胞内形成两种寄生虫。在这里，我们证明弓形虫中 CDPK7 蛋白的敲低会导致寄生虫分裂的明显缺陷和主要的生长缺陷，而它对于运动、出口和微线体胞吐作用是可有可无的。在cdpk7耗尽的寄生虫中，总体DNA含量没有受损，但出芽的子细胞的极性以及参与细胞分裂的几个亚细胞结构或蛋白质的命运受到影响，例如中心体和着丝粒。总的来说，我们的数据表明 CDPK7 对于正确维持内生发生所需的中心体完整性至关重要。我们的研究结果首次深入了解了钙依赖性信号传导在寄生虫繁殖中的可能作用，以及其在入侵和逃逸中的更广泛探索的作用。
Apicomplexan parasites express various Calcium-Dependent Protein Kinases (CDPKs), and some of them play essential roles in invasion and egress. Five of the six CDPKs conserved in most Apicomplexa have been studied at the molecular and cellular levels in Plasmodium species and/or in Toxoplasma gondii parasites, but the function of CDPK7 was so far uncharacterized. In T. gondii, during intracellular replication, two parasites are formed within a mother cell through a unique process called endodyogeny. Here we demonstrate that the knock-down of CDPK7 protein in T. gondii results in pronounced defects in parasite division and a major growth deficiency, while it is dispensable for motility, egress and microneme exocytosis. In cdpk7-depleted parasites, the overall DNA content was not impaired, but the polarity of daughter cells budding and the fate of several sub-cellular structures or proteins involved in cell division were affected, such as the centrosomes and the kinetochore. Overall, our data suggest that CDPK7 is crucial for proper maintenance of centrosome integrity required for the initiation of endodyogeny. Our findings provide a first insight into the probable role of calcium-dependent signalling in parasite multiplication, in addition to its more widely explored role in invasion and egress.
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弓形虫 IMC 蛋白的一个新家族在协调寄生虫分裂中表现出层次结构和功能。
影响因子：
6.7
作者：
Beck, Josh R.;Rodriguez-Fernandez, Imilce A.;de Leon, Jessica Cruz;Huynh, My-Hang;Carruthers, Vern B.;Morrissette, Naomi S.;Bradley, Peter J.
通讯作者：
Bradley, Peter J.
影响因子：
4.8
作者：
Ahmed, Anwar;Gaadhe, Kavita;Sharma, Pushkar
通讯作者：
Sharma, Pushkar
影响因子：
1.5
作者：
ACHBAROU, A;MERCEREAUPUIJALON, O;DUBREMETZ, JF
通讯作者：
DUBREMETZ, JF
DOI：
10.1016/s0022-5320(73)90063-4
发表时间：
1973-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
DUBREMETZ, JF
通讯作者：
DUBREMETZ, JF
影响因子：
4.8
作者：
Carruthers, VB;Sherman, GD;Sibley, LD
通讯作者：
Sibley, LD