Genome-Wide Identification and Characterization of Pectin Methylesterase Inhibitor Genes in Brassica oleracea.
Genome-Wide Identification and Characterization of Pectin Methylesterase Inhibitor Genes in Brassica oleracea.
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甘蓝果胶甲酯酶抑制剂基因的全基因组鉴定和表征
DOI：
10.3390/ijms19113338
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发表时间：
2018-10-26
影响因子：
5.6
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Liu T;Yu H;Xiong X;Yu Y;Yue X;Liu J;Cao J
果胶甲基酯酶抑制剂（PMEIs）调节果胶甲基酯酶（PMEs）的活性，从而控制果胶的甲酯化状态。然而，PMEI基因在经济上重要的蔬菜作物甘蓝中的作用知之甚少。在本研究中，95 B.鉴定了甘蓝PMEI（BoPMEI）基因。共检测到77个同线直向同源物对和10个串联重复簇，表明BoPMEI基因的扩增主要是通过全基因组三倍体（WGT）和串联重复（TD）进行的。在WGT后的二倍体化，BoPMEI基因优先保留根据基因平衡假说。大多数同源基因对在进化过程中经历了ω（Ka/Ks）小于1的纯化选择。通过表达谱分析鉴定了5个雄蕊特异的BoPMEI基因。结合对表达和进化的分析，我们推测在长期的进化过程中可能发生非功能化、亚功能化、新功能化和功能保守化。这项工作为B中PMEI基因的表征提供了见解。为进一步研究BoPMEI基因的功能奠定了基础。
The activities of pectin methylesterases (PMEs) are regulated by pectin methylesterase inhibitors (PMEIs), which consequently control the pectin methylesterification status. However, the role of PMEI genes in Brassica oleracea, an economically important vegetable crop, is poorly understood. In this study, 95 B. oleracea PMEI (BoPMEI) genes were identified. A total of 77 syntenic ortholog pairs and 10 tandemly duplicated clusters were detected, suggesting that the expansion of BoPMEI genes was mainly attributed to whole-genome triplication (WGT) and tandem duplication (TD). During diploidization after WGT, BoPMEI genes were preferentially retained in accordance with the gene balance hypothesis. Most homologous gene pairs experienced purifying selection with ω (Ka/Ks) ratios lower than 1 in evolution. Five stamen-specific BoPMEI genes were identified by expression pattern analysis. By combining the analyses of expression and evolution, we speculated that nonfunctionalization, subfunctionalization, neofunctionalization, and functional conservation can occur in the long evolutionary process. This work provides insights into the characterization of PMEI genes in B. oleracea and contributes to the further functional studies of BoPMEI genes.
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