The last rRNA methyltransferase of E. coli revealed: The yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA
The last rRNA methyltransferase of E. coli revealed: The yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA
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DOI：
10.1261/rna.034207.112
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发表时间：
2012-09-01
期刊：
影响因子：
4.5
通讯作者：
Dontsova, Olga A.
中科院分区：
文献类型：
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作者：
Golovina, Anna Y.;Dzama, Margarita M.;Dontsova, Olga A.
大肠杆菌的核糖体RNA （rRNA）含有24个甲基化残基。一组22个甲基转移酶负责23个残基的修饰已经描述过。在这里，我们报道了yhiR基因编码修饰23S rRNA核苷酸A2030的酶，这是最后一个甲基化的rRNA核苷酸，其修饰酶是未知的。YhiR更倾向于无蛋白的23S rRNA，而不是只含有部分50S亚基蛋白的核糖核蛋白颗粒，并且不会将组装的50S亚基甲基化。我们建议根据rRNA甲基转移酶命名法将yhiR基因重命名为rlmJ。yhiR敲除基因在各种生长条件和固定阶段的表型都非常温和，除了在厌氧条件下有小的生长优势。在缺乏23S rRNA核苷酸A2030甲基化的细胞中，只能观察到总大肠杆菌蛋白质组的微小变化。
The ribosomal RNA ( rRNA) of Escherichia coli contains 24 methylated residues. A set of 22 methyltransferases responsible for modification of 23 residues has been described previously. Herein we report the identification of the yhiR gene as encoding the enzyme that modifies the 23S rRNA nucleotide A2030, the last methylated rRNA nucleotide whose modification enzyme was not known. YhiR prefers protein-free 23S rRNA to ribonucleoprotein particles containing only part of the 50S subunit proteins and does not methylate the assembled 50S subunit. We suggest renaming the yhiR gene to rlmJ according to the rRNA methyltransferase nomenclature. The phenotype of yhiR knockout gene is very mild under various growth conditions and at the stationary phase, except for a small growth advantage at anaerobic conditions. Only minor changes in the total E. coli proteome could be observed in a cell devoid of the 23S rRNA nucleotide A2030 methylation.