Closely related poleroviruses depend on distinct translation initiation factors to infect Arabidopsis thaliana.
Closely related poleroviruses depend on distinct translation initiation factors to infect Arabidopsis thaliana.
复制标题
密切相关的杆病毒依赖于不同的翻译起始因子来感染拟南芥。
DOI：
10.1094/mpmi-07-12-0174-r
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Véronique Brault
中科院分区：
文献类型：
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作者：
C. Reinbold;S. Lacombe;V. Ziegler;D. Scheidecker;L. Wiss;Monique Beuve;C. Caranta;Véronique Brault
除了对真核生物mRNA的翻译至关重要外，翻译起始因子也是植物与病毒相互作用的关键组成部分。为了研究这些因素在极多病毒（蚜虫传播的、韧皮部受限的病毒）感染周期中的作用，研究人员在eIF4E和eIF4G家族翻译起始因子合成受损的拟南芥突变系中观察了三种极多病毒的积累。我们发现芜菁黄病毒（TuYV）在芜菁植物中的有效积累依赖于eIF (iso)4G1的存在，而甜菜轻度黄病毒（BMYV）和甜菜西部黄病毒- usa （BWYV-USA）则依赖于eIF4E1。这些因子在TuYV和BMYV的感染过程中发挥了作用，这些特异性因子与相关病毒的5'病毒基因组连接蛋白在酵母中直接相互作用。尽管潜在的分子机制尚不清楚，但本研究揭示了一种完全不可预见的情况，即属于同一属的密切相关病毒使用不同的翻译起始因子来有效感染拟南螺旋体。
In addition to being essential for translation of eukaryotic mRNA, translation initiation factors are also key components of plant-virus interactions. In order to address the involvement of these factors in the infectious cycle of poleroviruses (aphid-transmitted, phloem-limited viruses), the accumulation of three poleroviruses was followed in Arabidopsis thaliana mutant lines impaired in the synthesis of translation initiation factors in the eIF4E and eIF4G families. We found that efficient accumulation of Turnip yellows virus (TuYV) in A. thaliana relies on the presence of eIF (iso)4G1, whereas Beet mild yellowing virus (BMYV) and Beet western yellows virus-USA (BWYV-USA) rely, instead, on eIF4E1. A role for these factors in the infectious processes of TuYV and BMYV was confirmed by direct interaction in yeast between these specific factors and the 5' viral genome-linked protein of the related virus. Although the underlying molecular mechanism is still unknown, this study reveals a totally unforeseen situation in which closely related viruses belonging to the same genus use different translation initiation factors for efficient infection of A. thaliana.