Myosin heavy chain composition in rat laryngeal muscles after denervation
Myosin heavy chain composition in rat laryngeal muscles after denervation
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DOI：
10.1097/00005537-199808000-00023
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发表时间：
1998-08-01
期刊：
影响因子：
2.6
通讯作者：
Flint, PW
中科院分区：
文献类型：
--
作者：
Shiotani, A;Flint, PW
目的：去神经支配对特定喉肌肌球蛋白重链（MHC）表达的影响，其特征在于使用凝胶电泳。观察到的MHC组成的时间变化，然后将被用作生物标志物的研究，旨在制定战略喉神经再支配和基因治疗。研究设计：使用成年大鼠模型进行喉麻痹的动物研究。方法：在麻醉大鼠上，分离左侧喉返神经和上级喉神经。动物存活7、14、28、90和180天。对动物实施安乐死，切除甲杓肌（TA）、声带肌（VOC）、环杓后肌（PCA）、环杓外侧肌（LCA）和环甲肌（CT）。每一块肌肉进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和密度测量，以确定MHC纤维类型的组成。结果：描述了每种喉肌相对MHC组成的变化。在一般情况下，IIB型减少和IIA和IIX增加后，去神经。失神经支配条件下IIL的表达是可变的，I型中的相对变化最小。结论：这项研究支持以前的工作，使用大鼠比目鱼肌中IIA/IIX的表达是有利的条件下，神经肌肉活动减少，相反，IIB的表达是活动依赖性的。I型的表达似乎是独立的神经活动。将进行进一步的研究，以量化MHC组分的表达和研究调节表达的因素。肌球蛋白重链，SDS-PAGE，去神经支配，大鼠喉，喉返神经。
Objectives: The effects of denervation on myosin heavy chain (MHC) expression in specific laryngeal muscles are characterized using gel electrophoresis. Observed temporal changes in MHC composition will then be used as a biologic marker in studies designed to develop strategies for laryngeal reinnervation and gene therapy. Study Design: Animal study using an adult rat model for laryngeal paralysis. Methods: In anesthetized rats the left recurrent and superior laryngeal nerve were divided. Animals were survived for 7, 14, 28, 90, and 180 days. Animals were euthanized and the thyroarytenoid (TA), vocalis (VOC), posterior cricoarytenoid (PCA), lateral cricoarytenoid (LCA), and cricothyroid (CT) muscle excised. Each muscle was processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and densitometric measurements were obtained to determine composition of MHC fiber types. Results: The changes in relative MHC composition are described for each specific laryngeal muscle. In general, a decrease in type IIB and an increase in IIA and IIX are seen after denervation. Expression of IIL in the denervated condition is variable and the relative change in type I is minimal. Conclusion: This study supports previous work using rat soleus muscle in which IIA/IIX expression is favored in conditions with decreased neuromuscular activity, and conversely, IIB expression is activity dependent. Expression of type I appears to be independent of neural activity. Further study will be undertaken to quantify expression of MHC components and to study factors modulating expression. Rey Words: Myosin heavy chain, SDS-PAGE, denervation, rat larynx, recurrent laryngeal nerve.