Picoamperometric detection of glucose at ultrasmall platinum-based biosensors: Preparation and characterization
Picoamperometric detection of glucose at ultrasmall platinum-based biosensors: Preparation and characterization
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DOI：
10.1021/ac026438u
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发表时间：
2003-07-15
影响因子：
7.4
通讯作者：
Luong, JHT
中科院分区：
文献类型：
--
作者：
Hrapovic, S;Luong, JHT
介绍了一种构建具有良好重现性的葡萄糖生物传感器的简单方法。经过电化学刻蚀后，刻蚀的铂微电极的传感尖端用合成橡胶浸渍涂层绝缘。然后对绝缘层进行热固化，如扫描电子显微镜所证实的那样，在蚀刻的铂尖的末端形成一个小的裸露区域。苯酚和2-烯丙基苯酚的电聚合形成了额外的绝缘层，有效地将葡萄糖氧化酶(GOx)保留在电极的传感尖端。在循环伏安测量的基础上，生物传感器针尖的表观半径估计在10到500 nm之间，这取决于GOx的负载。以最佳GOx浓度(10 mg/mL)制备的葡萄糖生物传感器的工作稳定性和储存稳定性均在3周以上，其电流响应范围与S相似，检测下限为20微米，重现性良好。
A simple method is described for the construction of a glucose biosensor with good reproducibility. After electrochemical etching, the sensing tip of an etched platinum microelectrode was insulated using a synthetic rubber dip coating. The insulating layer was then heat-cured, leading to a small exposed area at the very end of the etched Pt tip, as confirmed by scanning electron microscopy. Phenol and 2-allylphenol were electropolymerized to form an extra insulating layer that effectively retained glucose oxidase (GOX) on the sensing tip of the electrode. On the basis of cyclic voltammetry measurements, the apparent radius of the biosensor tip was estimated to be between 10 and 500 nm, depending on GOX loading. With operational and storage stabilities over 3 weeks, the glucose biosensor prepared using optimal GOX concentration (10 mg/mL) exhibited a picoamperometric current response within similar to2 s and a detection limit of 20 muM with excellent reproducibility.