Effects of pH on biomass, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture.
Effects of pH on biomass, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture.
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pH 对连续培养中生长的三种皮肤丙酸杆菌的生物量、最大比生长速率和胞外酶产量的影响。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
W. Cunliffe
中科院分区：
文献类型：
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作者：
J. Greenman;K. T. Holland;W. Cunliffe
三种皮肤丙酸杆菌（痤疮丙酸杆菌、鸟丙酸杆菌和颗粒丙酸杆菌）使用不同 pH 值的半合成培养基在恒化器中生长。痤疮丙酸杆菌在 pH 4.5 和 7.5 之间生长，avidum 和颗粒丙酸杆菌在 pH 5.0 和 8.0 之间生长。三个物种的最高 mumax 为 pH 6.0。痤疮丙酸杆菌和 avidum 丙酸杆菌在 pH 6.0 时获得最大生物量，颗粒丙酸杆菌在 pH 7.5 时获得最大生物量。当考虑到酶的变性时，在整个 pH 生长范围内都会产生细胞外酶。然而，由于酶在低或高 pH 值下会变性，因此在较窄的 pH 范围内发现了酶的可检测活性。除了颗粒丙酸杆菌的透明质酸裂解酶（pH 6.0 至 7.0）以及痤疮丙酸杆菌和 avidum 的蛋白酶（pH 5.0 至 7.5）的蛋白酶产生外，酶的最高产量出现在 pH 值 5.0 至 6.0 之间。痤疮丙酸杆菌产生脂肪酶、透明质酸裂解酶、磷酸酶和蛋白酶活性。爱维丙酸杆菌产生脂肪酶和蛋白酶活性。颗粒丙酸杆菌产生脂肪酶和透明质酸裂解酶。
Three cutaneous propionibacteria, Propionibacterium acnes, Propionibacterium avidum and Propionibacterium granulosum, were grown in chemostats using semi-synthetic medium at various pH values. Growth occurred between pH 4.5 and 7.5 for P. acnes and pH 5.0 and 8.0 for P. avidum and P. granulosum. The highest mumax was at pH 6.0 for the three species. Maximum biomass production was obtained at pH 6.0 for P. acnes and P. avidum and at pH 7.5 for P. granulosum. Extracellular enzyme production occurred over the entire pH growth range when denaturation of the enzymes was taken into account. However, detectable activity of the enzymes was found in a narrower range of pH due to the denaturation of the enzymes at low or high pH values. The highest production of enzymes occurred at pH values between 5.0 and 6.0, apart from the production of hyaluronate lyase of P. granulosum (pH 6.0 to 7.0) and the proteinase of P. acnes and P. avidum (pH 5.0 to 7.5). Propionibacterium acnes produced a lipase, hyaluronate lyase, phosphatase and proteinase activity. Propionibacterium avidum produced a lipase and proteinase activity. Propionibacterium granulosum produced a lipase and hyaluronate lyase.