Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly
Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly
复制标题
DOI：
10.1073/pnas.95.7.3743
复制
发表时间：
1998-03-31
影响因子：
11.1
通讯作者：
Collins, FH
中科院分区：
文献类型：
--
作者：
Jasinskiene, N;Coates, CJ;Collins, FH
埃及伊蚊（Aedes aegypti）是世界上最重要的黄热病和登革热病毒的传播媒介。目前正在进行的工作是通过遗传改变野生埃及伊蚊种群支持病毒复制的能力来控制这些病毒的传播。本文报道的生殖系转化系统构成了这一控制策略实施的一个主要进展。使用携带4.7 kb基因组DNA片段的修饰的爱马仕转座子转化埃及伊蚊的白眼受体菌株，该片段包括果蝇朱砂（cn）基因的野生型等位基因。用该构建体微感染前胚盘期蚊胚，50%的G（0）成虫出现眼睛颜色。回收了三个转化的家系，每个家系都是由携带cn（+）转座子的独立插入事件产生的。cn（+）基因作为半显性转基因起作用，并以孟德尔比率分离。爱马仕显示出作为高效、可遗传、和稳定的转化。
The mosquito Aedes aegypti is the world's most important vector of yellow fever and dengue viruses, Work is currently in progress to control the transmission of these viruses by genetically altering the capacity of wild Ae, aegypti populations to support virus replication. The germ-line transformation system reported here constitutes a major advance toward the implementation of this control strategy, A modified Hermes transposon carrying a 4.7-kb fragment of genomic DNA that includes a wild-type allele of the Drosophila melanogaster cinnabar (cn) gene was used to transform a white-eyed recipient strain of Ae, aegypti. Microinfection of preblastoderm mosquito embryos with this construct resulted in 50% of the emergent G(0) adults showing some color in their eyes, Three transformed families were recovered, each resulting from an independent insertion event of the cn(+)-carrying transposon, The cn(+) gene functioned as a semidominant transgene and segregated in Mendelian ratios, Hermes shows great promise as a vector for efficient, heritable, and stable transformation of this important mosquito vector species.