Expression Profiling of MYOZ1 Gene in Porcine Tissue and C2C12 Cells
Expression Profiling of MYOZ1 Gene in Porcine Tissue and C2C12 Cells
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MYOZ1 基因在猪组织和 C2C12 细胞中的表达谱
DOI：
10.3923/javaa.2011.1917.1921
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Cao, Jian-Hua
中科院分区：
文献类型：
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作者：
Liu, Yin;Zhou, Ke;Cao, Jian-Hua
Z盘的结构和功能与骨骼肌的正常生理表型和功能密切相关。Myozenin（MYOZ）蛋白是一种α-辅肌动蛋白和γ-细丝蛋白结合的Z盘球状蛋白，主要在骨骼肌中表达，可能参与Z盘的形成和功能。MYOZ 1基因在猪骨骼肌中的表达量显著高于其它组织。定量PCR分析表明，MYOZ 1基因在猪胚胎33天、胚胎65天和成体骨骼肌发育过程中的表达呈阶梯性递增。利用C2 C12细胞进行功能测定，结果表明MYOZ 1不参与细胞增殖和分化。
The structure and function of Z-discs were related strongly to the normal physiological phenotype and function of skeletal muscle. The protein Myozenin (MYOZ), an cc-actinin and gamma-filamin-binding Z-disc globular protein expressed predominantly in skeletal muscle was possibly involved in the confirmation and function of Z-discs. Here we described MYOZ1 gene which has a significantly higher expression level in skeletal muscle than several other tissues in porcine. Furthermore, MYOZ1 transcripts in three porcine development stages (embryonic 33 days, embryonic 65 days and adult) of skeletal muscle shown stepwise increasingly expression pattern by using quantitative PCR analysis. Functional assays were conducted using C2C12 cells and the results implied MYOZ1 was not involved in both of cellular proliferation and differentiation.