Spontaneous deleterious mutation in Arabidopsis thaliana
Spontaneous deleterious mutation in Arabidopsis thaliana
复制标题
DOI：
10.1073/pnas.96.20.11393
复制
发表时间：
1999-09-28
影响因子：
11.1
通讯作者：
Willis, JH
中科院分区：
文献类型：
--
作者：
Schultz, ST;Lynch, M;Willis, JH
有害突变的频率和选择影响是进化理论中的基本参数，然而它们还没有在植物物种中直接测量，为了估计这些数量，我们允许自发突变在一年生自交植物拟南芥的1,000个自交系中积累10代，并在一个共同的花园中测试0代和10代之间的适合度差异。突变系的发芽率、座果率和单果种子数每代下降不到1%，总适合度每代下降0.9%。所有性状的突变系中的系内方差都增加了。等效应模型的应用表明，基因组有害突变率向下倾斜为0.1，个体突变对总适应度的向上倾斜效应为20%。这种基因组有害突变率与开花植物中核苷酸替换率的估计、拟南芥的基因组大小以及在这种高度自交的植物物种中观察到的平衡近亲繁殖抑制是一致的。
The frequency and selective impact of deleterious mutations are fundamental parameters in evolutionary theory, yet they have not been directly measured in a plant species, To estimate these quantities, we allowed spontaneous mutations to accumulate for 10 generations in 1,000 inbred lines of the annual, self-fertilizing plant Arabidopsis thaliana and assayed fitness differences between generations 0 and 10 in a common garden. Germination rate, fruit set, and number of seeds per fruit each declined by less than 1% per generation in the mutation lines, and total fitness declined by 0.9% per generation. Among-line variances increased in the mutation lines for all traits. Application of an equal-effects model suggests a downwardly biased genomic deleterious mutation rate of 0.1 and a upwardly biased effect of individual mutations on total fitness of 20%. This genomic deleterious mutation rate is consistent with estimates of nucleotide substitution rates in flowering plants, the genome size of Arabidopsis, and the equilibrium inbreeding depression observed in this highly selfing plant species.