Centrosome reduction during mouse spermiogenesis
Centrosome reduction during mouse spermiogenesis
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DOI：
10.1006/dbio.1998.8947
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发表时间：
1998-11-15
影响因子：
2.7
通讯作者：
Schatten, G
中科院分区：
文献类型：
--
作者：
Manandhar, G;Sutovsky, P;Schatten, G
在小鼠受精过程中，精子不参与中心体的形成。为了确定功能性中心体减少的方式，我们研究了小鼠精子发生过程中中心体的变性。圆形精子细胞显示正常的中心体，由一对中心粒组成，沿着有含γ-微管蛋白的灶。然而，它们似乎并不组织微管。伸长的精子细胞在颈部区域显示γ-微管蛋白斑点，而微管从核周环组织起来，如乳突。电子显微镜研究，使用免疫金标记显示，γ-微管蛋白主要是本地化的中心粒附属从一个星形的微管发出。诺考达唑处理和逆转后，微管在细胞质中随机重新聚合。γ-微管蛋白从颈部区域解离，并在精子形成过程中被丢弃在残留体中。在精子发生的睾丸期，远端中心粒退化，而在附睾期，近端中心粒丢失。小鼠精子中心体蛋白和中心粒的丢失进一步证实了小鼠受精过程中中心体的母系遗传。(C)北京：科学出版社.
The sperm does not contribute the centrosome during murine fertilization. To determine the manner in which a functional centrosome is reduced, we have studied centrosome degeneration during spermiogenesis of mice. The round spermatids display normal centrosomes consisting of a pair of centrioles along with gamma-tubulin containing foci. However, they do not seem to organize microtubules. Elongating spermatids display gamma-tubulin spots in the neck region, while microtubules are organized from the perinuclear ring as the manchette. Electron microscopic studies using immunogold labeling revealed that gamma-tubulin is mainly localized in the centriolar adjunct from which an aster of microtubules emanates. Microtubules repolymerized randomly in the cytoplasm after nocodazole treatment and reversal. gamma-Tubulin dissociates from the neck region and is discarded in the residual bodies during spermiation. The distal centriole degenerates during testicular stage of spermiogenesis, while the proximal centriole is lost during epididymal stage. Loss of centrosomal protein and centrioles in mouse sperm further confirm the maternal inheritance of centrosome during murine fertilization. (C) 1998 Academic Press.