Si-Ni-San, a traditional Chinese prescription, and its active ingredient glycyrrhizin ameliorate experimental colitis through regulating cytokine balance
Si-Ni-San, a traditional Chinese prescription, and its active ingredient glycyrrhizin ameliorate experimental colitis through regulating cytokine balance
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四逆散是一种中药方剂，其活性成分甘草甜素通过调节细胞因子平衡来改善实验性结肠炎。
DOI：
10.1016/j.intimp.2009.08.017
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发表时间：
2009-11-01
影响因子：
5.6
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
--
作者：
Sun, Yang;Cai, Tian-Tian;Xu, Qiang
四逆散是一种中药方剂，经过数千年的临床实践，在治疗炎症性肠病方面发挥着重要作用，但其作用机制尚不清楚。在这项研究中，我们研究了四镍散及其成分甘草甜素对三硝基苯磺酸 (TNBS) 诱导的小鼠实验性结肠炎的治疗潜力，这是一种具有良好特征的克罗恩病小鼠模型。 Si-Ni-San 和甘草甜素显着改善 TNBS 诱发的结肠炎，降低死亡率并恢复体重。此外，四镍散和甘草酸剂量依赖性地降低宏观炎症评分、微观组织学评分和髓过氧化物酶活性。此外，Si-Ni-San 和甘草甜素还导致小鼠结肠中促炎细胞因子（包括 IFN-γ IL-12、TNF-α 和 IL-17）减少，而调节性细胞因子 IL-10 增加。值得注意的是，四逆散450mg/kg的治疗效果明显优于其所含甘草甜素10mg/kg的治疗效果。总之，四逆散和甘草甜素通过调节促炎和抗炎细胞因子的产生，显着改善 TNBS 诱导的小鼠结肠炎。 (C) 2009 Elsevier B.V. 保留所有权利。
Si-Ni-San, a traditional Chinese medicinal formula, exerts an important function in the treatment of inflammatory bowel diseases based upon thousands of years of clinical practice, but the underlying mechanism is still unclear. in this study, we investigated the therapeutic potential of Si-Ni-San and its ingredient glycyrrhizin in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis in mice, a well-characterized murine model for Crohn's disease. Si-Ni-San and glycyrrhizin significantly ameliorated TNBS-induced colitis with reduced mortality and recovery of body weights. In addition, Si-Ni-San and glycyrrhizin dose-dependently decreased macroscopic inflammation scores, microscopic histoligical scores, and myeloperoxidase activity. Furthermore, Si-Ni-San and glycyrrhizin caused a decrease in pro-inflammatory cytokines including IFN-gamma IL-12, TNF-alpha and IL-17 and an increase in regulatory cytokine IL-10 in colon of the mice. It should be noticed the therapeutic effect of Si-Ni-San at 450 mg/kg was much better than that of its contained content of glycyrrhizin at 10 mg/kg. In conclusion, Si-Ni-San and glycyrrhizin significantly ameliorated TNBS-induced colitis in mice through regulating pro- and anti-inflammtory cytokine production. (C) 2009 Elsevier B.V. All rights reserved.