Determination of Thiabendazole and 5-Hydroxythiabendazole in Livestock Foods by HPLC-UV
Determination of Thiabendazole and 5-Hydroxythiabendazole in Livestock Foods by HPLC-UV
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HPLC-UV法测定畜禽食品中噻菌灵和5-羟基噻菌灵
DOI：
10.3358/shokueishi.40.6_481
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Uchiyama
中科院分区：
文献类型：
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作者：
M. Kawasaki;T. Ono;M. Murayama;M. Toyoda;S. Uchiyama
噻菌灵 (TBZ) 是一种苯并咪唑抗菌剂，用作牲畜驱虫药。 5-羟基噻苯达唑 (5-OH TBZ) 是 TBZ 在动物体内羟基化形成的代谢物。在使用强碱性（pH 11.0）缓冲液的传统 TBZ 分析中，通过提取从动物中提取 5-OH TBZ 的回收率低至 50%。为了建立高回收率同时分析 TBZ 和 5-OH TBZ 的方法，我们研究了萃取缓冲液的最佳 pH 值、合适的萃取溶剂和净化柱。结果发现，缓冲液的最佳pH值为9.0，对于猪肌肉和肝脏，合适的溶剂是乙酸乙酯(AcOEt)，对于猪脂肪和牛奶，合适的溶剂是MeCN。在所研究的三种固相萃取 (SPE) 净化柱中，使用氧化铝 N (ALN) 柱实现了两种化学品的最高回收率。
Thiabendazole (TBZ) is a benzimidazole antibacterial agent used as an anthelminthic for livestock. 5-Hydroxythiabendazole (5-OH TBZ) is a metabolite formed by hydroxylation of TBZ in an animal's body. The recovery of 5-OH TBZ from animals by extraction in conventional TBZ analysis, which employs a strongly alkaline (pH 11.0) buffer, is as low as 50%. In order to establish a method for simultaneous analysis of TBZ and 5-OH TBZ with high recovery, we examined the optimum pH of a buffer for extraction, a suitable solvent for extraction, and a clean-up column. As a result, it was found that the optimum pH of the buffer was 9.0 and that suitable solvents were ethyl acetate (AcOEt) for pig muscle and liver, and MeCN for pig fat and cow's milk. Among the three types of solid phase extraction (SPE) clean-up columns investigated, the highest recovery for both chemicals was achieved by using an alumina N (ALN) column.