Sequence of IGF-I, IGF-II, and HGF expression in regenerating skeletal muscle
Sequence of IGF-I, IGF-II, and HGF expression in regenerating skeletal muscle
复制标题
DOI：
10.1007/s00418-004-0704-y
复制
发表时间：
2004-11-01
影响因子：
2.3
通讯作者：
Yamaguchi, T
中科院分区：
文献类型：
--
作者：
Hayashi, S;Aso, H;Yamaguchi, T
各种细胞因子被认为在肌肉再生中发挥作用，然而，这些细胞因子的相互作用和作用机制在很大程度上仍然未知。在这项研究中，我们调查的作用，肝细胞生长因子，胰岛素样生长因子-I，和胰岛素样生长因子-II在肌再生模型的骨骼肌，以及成肌细胞培养。RT-PCR分析显示，HGF和IGF-I的表达显着上调，在再生肌肉。相反，正常和再生肌肉之间的IGF-II表达没有显着差异。免疫组织化学分析表明，在肌肉再生的早期阶段，HGF主要由肌细胞表达。此外，HGF抑制成肌细胞形成肌管，但促进细胞增殖。此外，在肌肉再生的早期至中期，肌细胞表达IGF-I和IGF-II。在体外培养的成肌细胞中加入HGF可显著增加细胞数量。这些发现表明，这三种细胞因子在再生骨骼肌中具有多效性作用。
Various cytokines are thought to play a role in muscle regeneration, however, the interaction and mechanisms of action of these cytokines remains largely unknown. In this study, we investigated the role of HGF, IGF-I, and IGF-II during myogenesis using the regeneration model of skeletal muscle as well as myoblast culture. RT-PCR analysis revealed that HGF and IGF-I expressions were markedly upregulated, in regenerating muscle. In contrast, there was no significant difference in IGF-II expression between normal and regenerating muscle. Immunohistochemical analysis demonstrated that HGF was expressed mostly by myocytes during the early stages of muscle regeneration. Additionally, HGF inhibited the formation of myotubes by myoblasts, but promoted cellular proliferation. Otherwise, IGF-I and IGF-II were expressed by myocytes through the early to middle stages of muscle regeneration. The addition of HGF to myoblast growing in vitro significantly increased the number of cells. These findings indicate that these three cytokines have pleiotropic effects in regenerating skeletal muscle.