Enhancement of magnetic nanoparticle-mediated gene transfer to astrocytes by 'magnetofection': effects of static and oscillating fields
Enhancement of magnetic nanoparticle-mediated gene transfer to astrocytes by 'magnetofection': effects of static and oscillating fields
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DOI：
10.2217/nnm.09.109
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发表时间：
2010-02-01
期刊：
影响因子：
5.5
通讯作者：
Chari, Divya
中科院分区：
文献类型：
--
作者：
Pickard, Mark;Chari, Divya
目的：评估使用磁性纳米颗粒（MNP）转染移植用星形胶质细胞的可行性，并确定静态和振荡磁场是否可以增强转染效率。方法：星形胶质细胞转染用MNP功能与质粒编码的报告蛋白。转染效率进行了比较应用程序的静态领域和一种新的，振荡阵列系统在一定范围内的频率。在器官型小脑切片培养中测试转染细胞的移植潜力。结果：大鼠星形胶质细胞可以有效地转染MNP与应用静态/振荡场;后者的效果是频率依赖性的。转染的星形胶质细胞可以存活并在引入3D神经组织阵列后分化。结论：MNP载体可以安全有效地转染啮齿动物星形胶质细胞，并可以形成用于神经细胞移植的“多功能纳米平台”的基础。
Aims: To assess the feasibility of using magnetic nanoparticles (MNPs) to transfect astrocytes derived for transplantation and determine if transfection efficacy can be enhanced by static and oscillating magnetic fields. Methods: Astrocytes were transfected using MNPs functionalized with a plasmid encoding a reporter protein. Transfection efficacies were compared following application of static fields and a novel, oscillating array system at a range of frequencies. The transplantation potential of transfected cells was tested in organotypic cerebellar slice cultures. Results: Rat astrocytes can be efficiently transfected using MNPs with applied static/oscillating fields; the latter effect is frequency dependant. Transfected astrocytes could survive and differentiate following introduction into 3D neural tissue arrays. Conclusion: MNP vectors can safely and effectively transfect rodent astrocytes and could form the basis of a 'multifunctional nanoplatform' for neural cell transplantation.