Cell cycle dependence of boron uptake from two boron compounds used for clinical neutron capture therapy
Cell cycle dependence of boron uptake from two boron compounds used for clinical neutron capture therapy
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DOI：
10.1016/s0304-3835(02)00380-4
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发表时间：
2002-12-10
期刊：
影响因子：
9.7
通讯作者：
Nose, T
中科院分区：
文献类型：
--
作者：
Yoshida, F;Matsumura, A;Nose, T
在中子俘获疗法中，肿瘤细胞选择性地摄取硼是很重要的。在本研究中，我们用流式细胞术对处于G0/G1期和G2/M期的细胞进行分选，并用电感耦合等离子体原子发射光谱仪测定每一组分中的硼浓度。结果表明，与G0/G1期相比，在G2/M期，BPA和BPA具有更高的硼吸收速率。然而，这种差异在双酚A的情况下更加突出。在含有BPA的培养液中，G2/M：G0/G1比值随暴露时间的延长而降低，从而表明细胞周期对BPA摄取的依赖。这种肿瘤细胞摄取硼的异质性应该被考虑用于微剂量学。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
In neutron capture therapy, it is important that the boron is selectively uptaken by tumor cells. In the present study, we used flow cytometry to sort the cells in the G0/G1 phase and those in the G2/M phase, and the boron concentration in each fraction was measured with inductively coupled plasma atomic emission spectroscopy. The results revealed that sodium borocaptate and boronophenylalanine (BPA), were associated with higher rates of boron uptake in the G2/M than in the G0/G1 phase. However, the difference was more prominent in the case of BPA. The G2/M:G0/G1 ratio decreased as a function of exposure time in BPA containing culture medium, thereby indicating the cell cycle dependency of BPA uptake. Such heterogeneity of boron uptake by tumor cells should be considered for microdosimetry. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.