Kinetic analysis of active efflux of vincristine from multidrug-resistant P388 leukemia cells.
Kinetic analysis of active efflux of vincristine from multidrug-resistant P388 leukemia cells.
复制标题
多重耐药 P388 白血病细胞长春新碱主动流出的动力学分析。
DOI：
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Yuichi Sugiyama
中科院分区：
文献类型：
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作者：
Makoto Inaba;Tohru Watanabe;Yuichi Sugiyama
Kinetic analysis of vincristine transport in parental and multidrug-resistant P388 leukemia cells was attempted by indirect assessment of its efflux. Practically, the initial velocity and steady-state level of vincristine uptake by ATP-depleted cells, and its steady-state level in untreated cells, were measured. As a result, a saturable process of not only influx but also efflux of vincristine was observed for the first time with both cell lines, suggesting the existence of a carrier-mediated system for influx and efflux. With increasing extracellular drug concentrations, the contribution of the mediated transport to the total flux was decreased and that of the unsaturable process, that is, simple diffusion, was increased. It should be particularly noted that the Km and Vmax values of efflux in the resistant cells were significantly less and greater, respectively, than those of the sensitive cells, providing a biochemical basis for enhanced efflux as a mechanism of multidrug-resistance. No significant difference in kinetic parameters of vincristine influx and intracellular binding contributing to resistance was found between the two cell lines.
大鼠肝细胞质与谷胱甘肽的结合。
影响因子：
3.1
作者：
Sugiyama,Y;Kaplowitz,N
通讯作者：
Kaplowitz,N
细胞表面多胺结合蛋白的光亲和标记。
DOI：
10.1074/jbc.270.48.28705
发表时间：
1995
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Felschow,DM;MacDiarmid,J;Bardos,T;Wu,R;Woster,PM;Porter,CW
通讯作者：
Porter,CW
能量依赖性药物结合减少是人类白血病淋巴母细胞长春花生物碱耐药的机制。
影响因子：
3.6
作者：
Beck,WT;Cirtain,MC;Lefko,JL
通讯作者：
Lefko,JL