Noninvasive blood pressure measurement in mice using pulsed Doppler ultrasound
Noninvasive blood pressure measurement in mice using pulsed Doppler ultrasound
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DOI：
10.1016/s0301-5629(02)00746-9
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发表时间：
2003-01-01
影响因子：
2.9
通讯作者：
Hartley, CJ
中科院分区：
文献类型：
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作者：
Reddy, AK;Taffet, GE;Hartley, CJ
现有的老鼠尾部袖带压力装置使用尾流传感器，仅测量收缩压和平均压。我们开发了一种使用脉冲多普勒血流速度传感器和尾套获得小鼠收缩压和舒张压的方法，并通过同时从充满液体的导管获得的压力信号验证了该方法。将尾袖加压至搏动以上水平，完全闭塞尾动脉，然后逐渐松解。尾部血流再次出现的压力被记录为收缩压，尾部如何变为连续的压力被记录为舒张压。从健康小鼠(n=16)获得的尾袖压力与导管压力的回归分析显示高度相关(r(Sys)=0.95，r(Dia)=0.94，在p<；0.001)。Bland-Altman分析显示两种方法具有良好的一致性，收缩压(58~250 mm Hg)和舒张压(48~178 mm Hg)的平均差值分别为-13(+/-12 SD)mm Hg和3(+/-10 SD)mm Hg。第二组小鼠(n=20)尾部袖带血压的Bland-Altman曲线图显示，同一天重复测量之间的一致性很好，但不同日期测量之间的变异性更高。(C)2003年世界医学生物学超声联合会。
Existing tail-cuff pressure devices for mice use tail flow sensors that measure only systolic and mean pressure. We developed a method to obtain systolic and diastolic pressure in mice using a pulsed Doppler flow velocity sensor and a tail-cuff and validated the method against pressure signals obtained simultaneously from a fluid-filled catheter. The tail-cuff was pressurized to suprasystolic levels to completely occlude the tail artery and then released gradually. The pressure at which the tail flow reappeared was recorded as systolic and the pressure at which the tail How became continuous was recorded as diastolic. Regression analysis of tail-cuff pressures over catheter pressures obtained from healthy mice (n = 16) showed a high degree of association (r(sys) = 0.95, r(dia) = 0.94, both at p < 0.001). Bland-Altman analysis showed good agreement between the two methods, with a mean difference of - 13 ( +/- 12 SD) mmHg and 3 ( +/- 10 SD) mmHg in the systolic (58 to 250 mmHg) and diastolic (48 to 178 mmHg) pressure measurements, respectively. Bland-Altman plots of tail-cuff blood pressures of a second group of mice (n = 20) showed good agreement between repeated measurements obtained on the same day, but had higher variability between measurements made on different days. (C) 2003 World Federation for Ultrasound in Medicine Biology.