The mechanism of thyrotrophin action in relation to lipid metabolism in thyroid tissue.
The mechanism of thyrotrophin action in relation to lipid metabolism in thyroid tissue.
复制标题
促甲状腺激素与甲状腺组织脂质代谢相关的作用机制。
作者：
T. W. Scott;S. Mills;N. Freinkel
1.本文研究了促甲状腺素在体外对[（14）C]-葡萄糖、-甘油、-棕榈酸和-油酸掺入甲状腺组织脂质的影响。2.促甲状腺激素增加这些（14）C-标记的前体磷脂酰肌醇的具体纳入。3.促甲状腺激素也增加了（14）C放射性的比例，从标记的葡萄糖，甘油，棕榈酸酯和油酸酯纳入1，2-甘油二酯。4.甲状腺切片中加入促甲状腺素10 min，2小时后。用[（14）C]甘油预标记，也增加了掺入1，2-甘油二酯组分的（14）C放射性的比例。5.甲状腺组织与[1-（14）C]棕榈酸酯孵育后，促甲状腺素导致从磷脂酰肌醇和1，2-甘油二酯中分离的棕榈酸酯的比放射性增加2 - 3倍。相比之下，从胆碱和乙醇胺磷酸甘油酯、1，3-甘油二酯和甘油三酯中分离的棕榈酸酯的比放射性未被促甲状腺素增加。
1. The effects of thyrotrophin in vitro on the incorporation of [(14)C]-glucose, -glycerol, -palmitate and -oleate into the lipids of thyroid tissue were examined. 2. Thyrotrophin increased the incorporation of these (14)C-labelled precursors into phosphatidylinositol specifically. 3. Thyrotrophin also increased the proportion of (14)C radioactivity from labelled glucose, glycerol, palmitate and oleate incorporated into the 1,2-diglycerides. 4. The addition of thyrotrophin to thyroid slices for 10min., after 2hr. of prelabelling with [(14)C]glycerol, also increased the proportion of (14)C radioactivity incorporated into the 1,2-diglyceride fraction. 5. After incubation of thyroid tissue with [1-(14)C]palmitate, thyrotrophin caused a two- to three-fold increase in the specific radioactivity of palmitate isolated from phosphatidylinositol and 1,2-diglycerides. In contrast, the specific radioactivity of palmitate isolated from the choline and ethanolamine phosphoglycerides, 1,3-diglycerides and triglycerides was not increased by thyrotrophin.