Mechano-regulatory cellular behaviors of NIH/3T3 in response to the storage modulus of liquid crystalline substrates.
Mechano-regulatory cellular behaviors of NIH/3T3 in response to the storage modulus of liquid crystalline substrates.
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DOI：
10.1016/j.jmbbm.2015.11.005
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发表时间：
2016-04
影响因子：
3.9
通讯作者：
Yang Chen;Lei Wang;Hao Huang;R. Tan;Jupeng Zhao;Shenyu Yang;R. Zeng;Haoming Wu;Jiaqing Zhang;Bin Yu;M. Tu
中科院分区：
文献类型：
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作者：
Yang Chen;Lei Wang;Hao Huang;R. Tan;Jupeng Zhao;Shenyu Yang;R. Zeng;Haoming Wu;Jiaqing Zhang;Bin Yu;M. Tu
基质硬度的程度已被证明在调节细胞行为中占主导地位。以前的研究使用弹性体或水凝胶等基质来了解细胞行为。在此，液晶矩阵，类似于生物膜和粘弹性的可移动的形态与可调的存储模量的评价模量驱动的细胞行为的制造。我们的研究结果表明，NIH/3 T3细胞表现出超敏反应的液晶基板的存储模量的变化，在附着，扩展，增殖和活力，极化，细胞周期和凋亡，以及机械转导相关的信号分子，包括FAK，桩蛋白和ERK的活性。具有12，312 Pa和7228 Pa的中间储能模量的辛基羟丙基纤维素基材（OPC-1-5）（分别为OPC-2和OPC-3）可以提供更有利的粘附条件，导致更大的铺展面积、更细长的形态和可能通过paxillin-ERK途径的更高的增殖速率，而具有最高或最低储能模量的基底（16，723 Pa，OPC-1;和41 Pa，OPC-5）似乎不利于细胞生长。我们的研究为更好地设计生物工程细胞基质提供了模块驱动细胞行为机制的见解。
The extent of substrate stiffness has been shown to be predominant in regulating cellular behaviors. Previous studies have used matrices such as elastomers or hydrogels to understand cell behavior. Herein, liquid crystalline matrices that resemble movable morphology of biomembrane and viscoelasticity were fabricated with tunable storage modulus for the evaluation of the modulus-driven cell behaviors. Our results demonstrated that NIH/3T3 cells showed a hypersensitive response to the storage modulus of liquid crystalline substrates by the alteration in attachment, spreading, proliferation and viability, polarization, cell cycle and apoptosis, and activity of mechano-transduction-related signal molecules including FAK, paxillin and ERK. The octyl hydroxypropyl cellulose substrates (OPC-1-5) with intermediate storage modulus of 12,312 Pa and 7228 Pa (OPC-2 and OPC-3 respectively) could provide more beneficial adhesion conditions leading to a larger spreading area, more elongated morphology and higher proliferation rates possibly through paxillin-ERK pathway, whereas the substrates with the highest or lowest storage modulus (16,723 Pa, OPC-1; and 41 Pa, OPC-5, respectively) appeared unfavorable for cell growth. Our study provides insights into the mechanism of modulus-driven cellular behaviors for better design of bioengineered cell substrates.