Association of interleukin-1B and interleukin-1RN polymorphisms with gastric cancer in a high-risk population of Costa Rica
Association of interleukin-1B and interleukin-1RN polymorphisms with gastric cancer in a high-risk population of Costa Rica
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DOI：
10.1007/s10238-005-0082-3
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发表时间：
2005-12-01
影响因子：
4.6
通讯作者：
Sierra, R
中科院分区：
文献类型：
--
作者：
Alpízar-Alpízar, W;Pérez-Pérez, GI;Sierra, R
一些危险因素与胃癌有关，其中包括幽门螺杆菌感染。这种细菌产生炎症，其程度取决于细菌菌株和宿主反应的严重程度。炎症反应涉及一个复杂的细胞因子网络。最近，编码白介素-1 β (IL-1B)、白介素- 1ra （ILRN）和白介素-10的基因多态性与胃癌风险增加有关。为了确定IL-1B、IL-1RN和IL-10多态性与哥斯达黎加高危人群胃癌的关系，我们分析了58名胃癌患者、99名对照组和41名根据日本分类分为I组或II组的患者的纯化DNA。采用PCR、PCR- rflp和焦磷酸测序分析进行基因分型。在研究人群中，我们没有发现IL-1B-31、IL-1B-511和IL-10多态性与发生胃癌的风险有任何关联。IL-1B+3954T/-的携带者发生胃癌的风险增加（OR 3.7; 95%CI: 1.34-10.2）。我们还发现IL-1RN等位基因2杂合子发生胃癌的风险增加（OR 2.94; 95%CI: 1.09-7.93）。这是IL-1B+3954首次与胃癌相关。这是第一个试图描述IL-1B， IL-1RN和IL-10基因多态性在胃癌高风险的美国国家之一中的作用的研究之一。需要对美国国家进行进一步的调查。
Several risk factors have been associated with gastric cancer, among them Helicobacter pylori infection. This bacterium yields inflammation, the degree of which depends on the bacterial strain and the severity of the host response. The inflammatory response involves a complex cytokine network. Recently, polymorphisms of the genes coding for interleukin-1 beta (IL-1B), interleukin-1Ra (ILRN) and interleukin-10 have been associated with an increased risk of gastric cancer. In order to determine the association of the IL-1B, IL-1RN and IL-10 polymorphisms with gastric cancer in a high-risk Costa Rican population, we analysed purified DNA of 58 gastric cancer patients, 99 controls and 41 patients classified as group I or II, according to the Japanese classification. Genotyping was carried out by PCR, PCR-RFLP and pyrosequencing analysis. We did not find any association of the IL-1B-31, IL-1B-511 and IL-10 polymorphisms with the risk for developing gastric cancer in the studied population. Carriers of the IL-1B+3954T/- had an increased risk for developing gastric cancer (OR 3.7; 95%CI: 1.34-10.2). Also we found an increased risk for developing gastric cancer for allele 2 heterozygotes of the IL-1RN (OR 2.94; 95%CI: 1.09-7.93). This is the first time that IL-1B+3954 has been associated with gastric cancer. This is one of the first studies trying to describe the role played by IL-1B, IL-1RN and IL-10 genetic polymorphisms in gastric cancer in one of the highest risk American countries. Further investigation on American countries is needed.