Buccal epithelium Expanded and Encapsulated in Scaffold-Hybrid Approach to Urethral Stricture (BEES-HAUS) procedure: A novel cell therapy-based pilot study
Buccal epithelium Expanded and Encapsulated in Scaffold-Hybrid Approach to Urethral Stricture (BEES-HAUS) procedure: A novel cell therapy-based pilot study
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DOI：
10.1111/iju.13852
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发表时间：
2019-02-01
影响因子：
2.6
通讯作者：
Abraham, Samuel J. K.
中科院分区：
文献类型：
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作者：
Vaddi, Surya P.;Reddy, Vijaya B.;Abraham, Samuel J. K.
目的描述一种新的基于细胞的内窥镜技术的可行性，使用颊上皮，扩张和封装在一个热可逆凝胶聚合物支架治疗尿道狭窄。方法6例男性尿道球部狭窄患者，尿道长度2.0 ~ 3.5cm。从一个小的颊粘膜活检的自体颊上皮细胞分离，培养和封装在热可逆凝胶聚合物支架，并植入狭窄部位后，广泛的内窥镜尿道切开术。结果所有患者均排尿通畅，平均最大尿流率24 mL/s。6个月时进行的尿道镜检查显示尿道切开部位的粘膜健康。然而，6例患者中有2例分别在18个月和24个月时复发。结论内镜下颊黏膜上皮扩张包埋支架-混合尿道成形术（BEES-HAUS）是一种可行的开放式替代颊黏膜尿道成形术。这是可能实现的好处开放替代颊尿道成形术与这种内窥镜技术。
Objectives To describe the feasibility of a novel cell-based endoscopic technique using buccal epithelium, expanded and encapsulated in a thermoreversible gelation polymer scaffold for the treatment of urethral stricture. Methods Six male patients with bulbar urethral stricture ranging from 2.0 to 3.5 cm in length were included in this pilot study. Autologous buccal epithelial cells from a small buccal mucosal biopsy were isolated, cultured and encapsulated in thermoreversible gelation polymer scaffold, and were implanted at the stricture site after a wide endoscopic urethrotomy. Results All the patients voided well, with a mean peak flow rate of 24 mL/s. Urethroscopy carried out at 6 months showed healthy mucosa at the urethrotomy site. However, two of the six patients had recurrence at 18 and 24 months, respectively. Conclusions This endoscopic-based Buccal epithelium Expanded and Encapsulated in Scaffold-Hybrid Approach to Urethral Stricture (BEES-HAUS) technique is a promising alternative for the open substitution buccal graft urethroplasty. It is possible to achieve the benefits of open substitution buccal urethroplasty with this endoscopic technique.