Inter and intra-population variability of Jatropha curcas (L.) characterized by RAPD and ISSR markers and development of population-specific SCAR markers
Inter and intra-population variability of Jatropha curcas (L.) characterized by RAPD and ISSR markers and development of population-specific SCAR markers
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DOI：
10.1007/s10681-007-9387-5
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发表时间：
2007-09-01
期刊：
影响因子：
1.9
通讯作者：
Sujatha, M.
中科院分区：
文献类型：
--
作者：
Basha, S. D.;Sujatha, M.
小桐子（Jatrophacurcas）是一种具有多种用途的油料作物，作为生物能源作物具有相当大的潜力。目前的调查已进行评估的遗传多样性的程度，在一个有代表性的一组42个加入J. curcas涵盖不同的作物种植区在印度沿着与无毒的基因型从墨西哥作为一个前奏，利用有前途的和遗传上不同的材料在育种计划。分子多态性为42.0%，400个快速引物和33.5%，100个ISSR引物之间的加入，表明适度的遗传变异水平在印度种质。RAPID多态性的群体内变异率为64.0%，与群体间变异率相当。多态性ISSR标记已被确定，可以区分印度加入墨西哥基因型，其中两个转化为SCAR标记。SCAR引物对ISPJ 1在所有印度人群中扩增出543 bp的片段，而具有1，096 bp特异性扩增子的ISPJ 2特异于墨西哥基因型。已经鉴定了来自喀拉拉邦的加入物（2个RAPID标记）、来自拉贾斯坦邦的Neemuch-1（1个RAPID和1个ISSR标记）和来自墨西哥的无毒基因型（17个RAPD和4个ISSR标记）的群体特异性条带，其用作基因分型中的诊断标记。该研究表明，迫切需要通过引入具有更广泛地理背景的种质来扩大小桐子种质的遗传基础。
Jatropha curcas (Euphorbiaceae) is an oil-bearing species with multiple uses and considerable potential as a bioenergy crop. The present investigation has been undertaken to assess the extent of genetic diversity in a representative set of 42 accessions of J. curcas encompassing different crop growing regions in India along with a non-toxic genotype from Mexico as a prelude for utilization of promising and genetically divergent materials in the breeding programmes. Molecular polymorphism was 42.0% with 400 RAPID primers and 33.5% with 100 ISSR primers between accessions indicating modest levels of genetic variation in the Indian germplasm. The within-population variation based on RAPID polymorphism was 64.0% and was on par with the inter-population variation. Polymorphic ISSR markers have been identified that could differentiate the Indian accessions from the Mexican genotype and two of them were converted to SCAR markers. The SCAR primer pair ISPJ1 amplified a 543 bp fragment in all the Indian populations, while ISPJ2 with a specific amplicon of 1,096 bp was specific to the Mexican genotype. Population-specific bands have been identified for the accession from Kerala (2 RAPID markers), Neemuch-1 from Rajasthan (I each of RAPID and ISSR markers) and the non-toxic genotype from Mexico (17 RAPD and 4 ISSR markers), which serve as diagnostic markers in genotyping. The study indicates an immediate need for widening the genetic base of J. curcas germplasm through introduction of accessions with broader geographical background.