RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection.
RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection.
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DOI：
10.3390/ijms18020234
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发表时间：
2017-02-10
影响因子：
5.6
通讯作者：
Xia Q
中科院分区：
文献类型：
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作者：
Xing D;Yang Q;Jiang L;Li Q;Xiao Y;Ye M;Xia Q
家蚕是一种重要的经济物种。白僵菌是蚕业中主要的真菌病害，了解家蚕对白僵菌感染的反应具有重要意义。本文采用RNA-seq方法研究了两种家蚕品系“好月”（HY，对球孢白僵菌敏感）和“康8”（K8，对球孢白僵菌抗性）这些反应的分子机制。每个菌株收集3个浸泡处理的生物重复、2个注射处理的生物重复和3个未处理的对照，共生成16个文库进行测序。鉴定了处理过的样品与未处理过的对照以及两个家蚕品系之间的差异表达基因（DEGs）。两菌株的DEGs和富集的京都基因与基因组百科全书（KEGG）通路表现出明显差异。一些编码角质层蛋白、丝氨酸蛋白酶抑制剂（SPI）和抗菌肽（AMP）的基因以及参与毒素解毒的药物代谢途径被认为与K8对球孢白僵菌的抗性有关。这些结果揭示了两个家蚕品系对球孢白僵菌感染的抗性和敏感性，为提高家蚕对球孢白僵菌抗性的分子育种提供了路线图。
The silkworm Bombyx mori is an economically important species. White muscardine caused by Beauveria bassiana is the main fungal disease in sericulture, and understanding the silkworm responses to B. bassiana infection is of particular interest. Herein, we investigated the molecular mechanisms underlying these responses in two silkworm strains Haoyue (HY, sensitive to B. bassiana) and Kang 8 (K8, resistant to B. bassiana) using an RNA-seq approach. For each strain, three biological replicates for immersion treatment, two replicates for injection treatment and three untreated controls were collected to generate 16 libraries for sequencing. Differentially expressed genes (DEGs) between treated samples and untreated controls, and between the two silkworm strains, were identified. DEGs and the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the two strains exhibited an obvious difference. Several genes encoding cuticle proteins, serine proteinase inhibitors (SPI) and antimicrobial peptides (AMP) and the drug metabolism pathway involved in toxin detoxification were considered to be related to the resistance of K8 to B. bassiana. These results revealed insight into the resistance and susceptibility of two silkworm strains against B. bassiana infection and provided a roadmap for silkworm molecular breeding to enhance its resistance to B. bassiana.